Elucidating assembly, ligand binding and gating of heterotetrameric CNG channels by FRET (B01)

• Subject Area: Biophysics
• Term: from 2015 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 258780946

Cyclic nucleotide-gated channels (CNG) constitute the last step of the phototransduction cascade. In this project optical and electrophysiological techniques will be used to elucidate elementary steps in the activation of the channel, such as the sequence of ligand binding to the individual subunits and their conformational rearrangement. Another part of the project will focus on the mechanisms by which the cell ensures that the channels are assembled of subunits in a fixed stoichiometry.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 166: High-end light microscopy elucidates membrane receptor function - ReceptorLight
• Applicant Institution: Friedrich-Schiller-Universität Jena
• Project Heads: Professor Dr. Christoph Biskup; Dr. Vasilica Nache