Spatial organization and dynamics of GPCR signaling as revealed by single-molecule and superresolution microscopy (C01)

• Subject Area: Pharmacology
• Term: from 2015 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 258780946

G protein-coupled receptors (GPCRs) constitute the largest family of receptors for hormones and neurotransmitters and represent major pharmacological targets. In this project, we are using high-end optical methods such as single-molecule microscopy and superresolution imaging (dSTORM) to investigate the organization of GPCRs in dynamic nanodomains on the surface of living cells. We expect these experiments to lead to a deeper understanding of the basic mechanisms of GPCR signaling, which might pave the way to innovative and more specific pharmacological therapies for a wide range of human diseases.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 166: High-end light microscopy elucidates membrane receptor function - ReceptorLight
• Applicant Institution: Friedrich-Schiller-Universität Jena
• Co-Applicant Institution: Julius-Maximilians-Universität Würzburg
• Project Head: Professor Dr. Davide Calebiro