Regulatory architecture and variation of gene expression in wild and domesticated barley
Plant Breeding and Plant Pathology
Final Report Abstract
Barley, like other crops, has experienced a series of genetic changes that have impacted its architecture and growth habit to suit the needs of humans, termed the domestication syndrome. Domestication also resulted in a concomitant bottleneck that reduced sequence diversity in genes and regulatory regions. Little is known about regulatory changes resulting from domestication in barley. We used RNA-seq to examine allele-specific expression (ASE) in hybrids between wild and domesticated barley. Our results show that most genes have conserved regulation. In contrast to studies of allele specific expression in interspecific hybrids, we find almost a complete absence of trans effects. We also find that cis regulation is largely stable in response to short-term cold stress. Our study has practical implications for crop improvement using wild relatives. Genes regulated in cis are more likely to be expressed in a new genetic background at the same level as in their native background.
Publications
- Regulatory architecture and variation of gene expression in wild and domesticated barley. 4th International Symposium on Genomics of Plant Genetic Resources, Giessen, Germany, Sep 4th 2017
MW Haas
- The contribution of cis- and trans-acting variants to gene regulation in wild and domesticated barley, Plant and Animal Genome Conference, San Diego, USA, January 16th 2018
MW Haas
- Domestication and crop evolution of wheat and barley: Genes, genomics, and future directions (2019). Journal of Integrative Plant Biology. 61:204-225
Haas MW, Schreiber M, Mascher M
(See online at https://doi.org/10.1111/jipb.12737) - Linear modeling reveals a predominance of cis-over trans-regulatory effects in wild and domesticated barley (2019)
Haas MW, Himmelbach A, Mascher M
(See online at https://doi.org/10.1101/661926)
