SNARE complex function in Ca 2+ -triggered exocytosis (B16)

• Subject Area: Cell Biology
• Term: from 2006 to 2008
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 5481217

The SNARE proteins syntaxin 1, SNAP-25 and synaptobrevin 2 form the basal fusion machineryduring Ca -dependent exocytosis. According to a popular hypothesis, assembly of the ternarySNARE complex between apposed membranes leads to membrane fusion. We will investigatethe assembly pathway of the complex, by introducing destabilizing mutations into synaptobrevin2. In addition, we will use chimeric constructs between different SNAREs to search forspecializations that allow membrane fusion to be fast. All mutations/chimera will be investigated in a null background using fast electrophysiological techniques.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 523: Protein and Membrane Transport between Cellular Compartments
• Applicant Institution: Georg-August-Universität Göttingen
• Co-Applicant Institution: Georg-August-Universität Göttingen; Albrecht-von-Haller-Institut der Pflanzenwissenschaften; Abteilung Biochemie der Pflanze
• Project Head: Professor Dr. Jakob Balslev Soerensen