Molecular Mechanisms underlying selection of cargo, scission, and coat release of intracellular vesicular carriers

• Applicant: Professor Dr. Felix Wilhelm Theodor Wieland
• Subject Area: Biochemistry
• Term: from 2016 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 287901311

• Final Report Year: 2020

Final Report Abstract

In the project “Molecular Mechanisms underlying selection of cargo, scission, and coat release of intracellular vesicular carriers” we have investigated i) the structural basis for the COPI scission reaction mediated by Arf, and whether a general mechanism exists for membrane scission catalyzed by GTP-binding proteins, ii) the selectivity of different COPI and COPII coat protein isoforms for the uptake of protein cargo by analysing the proteomes of isotypic vesicles reconstituted with recombinant coat proteins from semi intact cells, and iii) how GTPase-activating proteins catalyze the removal of the COPI coat and possibly aid the conversion of polymerized coat proteins to their soluble form.

Publications

• (2017) 9Å structure of the COPI coat reveals that the Arf1 GTPase occupies two contrasting molecular environments. Elife 16;6, pii: e26691
  Dodonova S.O., Aderhold P., Kopp J., Ganeva I, Röhling S, Hagen WJH, Sinning I, Wieland F, Briggs JAG.
  (See online at https://doi.org/10.7554/eLife.26691)
• (2018) Assembly of COPI and COPII Vesicular Coat Proteins on Membranes. Ann.Rev. Biophys 47
  Béthune J and Wieland FT
  (See online at https://doi.org/10.1146/annurev-biophys-070317-033259)
• (2019) Proteomic Profiling of COPII and COPI Vesicles. Cell Reports 26, 250-265
  Adolf F, Rhiel M, Hesssling B, Gao Q, Hellweg A, Bethune J, Wieland FT
  (See online at https://doi.org/10.1016/j.celrep.2018.12.041)