The hemolysin A secretion machinery from E. coli (A01)

• Subject Area: Biochemistry
• Term: since 2016
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 267205415

We study a Type 1 secretion system (T1SS), which translocates the human pathogenic RTX toxin HlyA in one step across both membranes of E. coli. The project relates the dynamic assembly of the translocation complex with the molecular identity of the transport-competent components of this machinery. In a combined approach of in vitro and in vivo experiments the following key aspects will be addressed: (1) identification of switches and triggers involved in identity changes in T1SS (structural studies), (2) addressing the molecular interaction network and in vitro reconstitution of a T1SS (functional studies) and (3) analysis of cellular HlyA secretion and a detailed in vivo / in vitro correlation (cellular studies).

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1208: Identity and Dynamics of Membrane Systems - from Molecules to Cellular Functions
• Applicant Institution: Heinrich-Heine-Universität Düsseldorf
• Project Head: Professor Dr. Lutz Schmitt