Dynamics and trafficking of mGBP protein complexes within membranous compartments (A06)

• Subject Area: Immunology
• Term: since 2016
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 267205415

The GTPase family of mGBPs are crucial proteins in cell autonomous immunity against Toxoplasma gondii. We investigate the mechanisms involved in mGBP regulated control of T. gondii infection. For this, we study the molecular interaction of mGBPs with host- and parasite-derived proteins, that we identified within the CRC. Furthermore, we will define the molecular mechanisms of lipid and membrane binding by mGBPs by using synthetic membranes and by superresolution and electron microscopy of intracellular pathogen vacuole membranes. Finally, we will study the role of ISGylation of mGBPs in the context of T. gondii infection.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1208: Identity and Dynamics of Membrane Systems - from Molecules to Cellular Functions
• Applicant Institution: Heinrich-Heine-Universität Düsseldorf
• Project Heads: Dr. Daniel Degrandi; Professor Dr. Klaus Pfeffer