Elucidation of the dynamics of the autophagosomal membrane-associated protein GABARAP by NMR spectroscopy (B03)

• Subject Area: Structural Biology
• Term: from 2016 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 267205415

Although autophagy is one of the fundamental cellular protein degradation mechanisms, the regulation of autophagosomal membrane compartments is still not fully understood. Using state-of-the-art NMR spectroscopy in concert with other biophysical and biochemical techniques we will investigate the conformational dynamics and functional mechanism of GABARAP, a key autophagy-related protein which alternates between a soluble form in the cytosol and a membrane-anchored form to mediate membrane tethering and hemifusion during autophagosome biogenesis.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1208: Identity and Dynamics of Membrane Systems - from Molecules to Cellular Functions
• Applicant Institution: Heinrich-Heine-Universität Düsseldorf
• Project Head: Dr. Philipp Neudecker