Antibacterial activity of light triggered antibiotics against uropathogenic bacteria
Final Report Abstract
Unfortunate excessive use of antibiotics for the treatment of urinary tract infections has led to a considerable and alarming increase in the number of resistant bacteria in many countries. Destruction of multidrug-resistant pathogens via reactive oxygen species produced by photosensitizer (PS) of appropriate structure could represent a valuable alternative therapeutic modality for the treatment of urinary tract infections. Development of antimicrobial photodynamic therapy is highly dependent on the development of suitable PSs. Within this project, a promising general approach is proposed to enable a selective and specific binding of PS to polysaccharides as particularly attractive targets; polysaccharides are cell membrane constituents that have a crucial importance for the survival of Gram-negative bacteria and also one of the main components of the biofilm matrix. We could distinctly demonstrate that functionalization of PS with boronic acid groups can significantly increase the binding of PS to Gram-negative E. coli in planktonic and biofilm cultures and induce cell death upon irradiation. An additional advantage of boronic acid functionality is a possibility to anchor the tailor-made PS to poly(vinyl alcohol) and to fabricate a self-disinfecting coating. The current results provide new insights into the formation of dynamic coatings that may have a big impact in reducing the risk of device-associated infections.
Publications
- Boronic acid-functionalized photosensitizers: a strategy to target the surface of bacteria and implement active agents in polymer coatings. Angew. Chem. Int. Ed. 2017, 56, 10362–10366; Angew. Chem. 2017, 129, 10498–10502
A. Galstyan, R. Schiller, U. Dobrindt
(See online at https://doi.org/10.1002/anie.201703398) - Conjugated phthalocyanines as light driven antibiotics, Photodiagnosis and Photodynamic Therapy, 2017, 17, A55
A. Galstyan, D. Block, S. Niemenn, K. Riehemann, C.A. Strassert, A. Faust, M. Schäfers, U. Dobrindt
(See online at https://doi.org/10.1016/j.pdpdt.2017.01.124) - Gaining Access to Bacteria through (Reversible) Control of Lipophilicity. Chem. Eur. J. 2017
A. Galstyan, J. Putze, U. Dobrindt
(See online at https://doi.org/10.1002/chem.201704562) - Making and Breaking Barriers to Overcome Microbial Infections. Optical Molecular Probes, Imaging and Drug Delivery, 2017, OmW3D. 4
A Galstyan, R Schiller, U Dobrindt
(See online at https://doi.org/10.1364/OMP.2017.OmW3D.4)
