RNA-protein interactomics to read the yeast mRNP code

• Applicant: Dr. Falk Butter
• Subject Area: General Genetics and Functional Genome Biology ; Biochemistry
• Term: from 2016 to 2020
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 313087757

We here propose to use mass spectrometry to identify protein binding partners at the level of individual RNA folds and to define an RBP interactome network based on quantitative interactome data. We will use our streamlined RNA-protein interactomics approach to uncover RNA-binding proteins at phylogenetic supported mRNA folds and characterize RNA-dependent mRBP composition in S. cerevisiae. Besides uncovering systematically the functionality of these RNA readers in yeast for a systems perspective, our technique is scalable and widely applicable to any RNA structure and species. It thus can contribute to deciphering the mRNP code also in other model systems.

• DFG Programme: Priority Programmes
• Subproject of: SPP 1935: Deciphering the mRNP code: RNA-bound determinants of post-transcriptional gene regulation