Rapid ribonucleoprotein-dependent recruitment of mRNA to ribosomes in the high light acclimation of Arabidopsis thaliana
Final Report Abstract
Photosynthesis is the major driver of leaf mesophyll energization and the provider of reduced C-, N- and S-metabolites for cell metabolism including amino acids for protein synthesis. The project started out with the hypothesis and first evidence that changes in incident photosynthetic active radiation (PAR) affects recruitment and translation of mRNAs at ribosomes by retrograde signalling on a short time scale of a few minutes. Two types of experiments addressed and proved this hypothesis. Low light-acclimated plants were transferred to 100-fold increased PAR and changes in polysome-associated transcripts were compared with changes in total mRNAs. Indeed, within 10 min after low light- to high lighttransfer, the translatome was reorganized. Analysis of the preferentially polysome-associated transcript revealed photosynthesis-related transcripts being up-regulated exclusively at the transcriptome and stress-related transcripts being increased in total mRNA and polysome association. Motifs in 5’-untranslated regions were identified and cytosolic glyceraldehyde-3-phosphate dehydrogenase GAPC1/2 as mRNA binding partner. We then also found reorganization of the translatome during daily light transitions in the morning and evening within 10 min after the switch in illumination and this process was affected by the cytosolic glyceraldehyde-3-phosphate dehydrogenase GAPC1/C2 as shown in gapc1/2 knock down plants. Results from studying the ability of the GAPC2 to bind to cis-motifs in 5’-UTR and its regulation in vitro were in line with a derived working model where binding of GAPC2, possibly representative for other enzymes of central metabolism, participate in tuning the translational activity in mesophyll cells depending on the energization state and the metabolic requirements.
Publications
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Magnetic Tracking of Protein Synthesis in Microfluidic Environments—Challenges and Perspectives. Nanomaterials, 9(4), 585.
Wegener, Melanie; Ennen, Inga; Walhorn, Volker; Anselmetti, Dario; Hütten, Andreas & Dietz, Karl-Josef
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Toward an Integrated Understanding of Retrograde Control of Photosynthesis. Antioxidants & Redox Signaling, 30(9), 1186-1205.
Dietz, Karl-Josef; Wesemann, Corinna; Wegener, Melanie & Seidel, Thorsten
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ConCysFind: a pipeline tool to predict conserved amino acids of protein sequences across the plant kingdom. BMC Bioinformatics, 21(1).
Moore, Marten; Wesemann, Corinna; Gossmann, Nikolaj; Sahm, Arne; Krüger, Jan; Sczyrba, Alexander & Dietz, Karl-Josef
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Retrograde Control of Cytosolic Translation Targets Synthesis of Plastid Proteins and Nuclear Responses for High-Light Acclimation. American Geophysical Union (AGU).
Moore Marten, Smith Aaron B.; Wegener Melanie, Wesemann Corinna; Schmidtpott Sonja, Farooq Muhammad Ansar; Ganguly Diep Ray, Seidel Thorsten & Pogson Barry J., Dietz Karl-Josef
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The mutual interaction of glycolytic enzymes and RNA in post-transcriptional regulation. RNA, 28(11), 1446-1468.
Wegener, Melanie & Dietz, Karl-Josef
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Mechanisms and implications of light-induced regulation of translation in Arabidopsis thaliana. Dissertation, Universität Bielefeld
Wegener M.
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Mechanisms of selective translational control under short-term high light stress. Dissertation, Universität Bielefeld
Wesemann C.
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Reprogramming the translatome during daily light transitions as affected by cytosolic glyceraldehyde-3-phosphate dehydrogenases GAPC1/C2. Journal of Experimental Botany, 75(8), 2494-2509.
Wegener, Melanie; Persicke, Marcus & Dietz, Karl-Josef
