Signaling platforms and structures required for cell motility and guided cell migration

• Applicant: Professor Dr. Erez Raz
• Subject Area: Developmental Biology ; Cell Biology
• Term: from 2016 to 2024
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 316511463

• Final Report Year: 2024

Final Report Abstract

The aim of this project was to achieve a comprehensive understanding of the dynamic cellular interfaces associated with the transitions migratory cells perform, leading to cell motility and those characterizing migration towards directional cues in the environment. In addition, it was proposed to investigate the events leading to loss of cell polarity, for example when cells need to change their migration direction, or stop at the target. As described in the report, we have gained detailed description and experimental information that provide mechanistic understanding of the process of gain of cellular polarity along the front-rear axis, the components and features allowing the cells to migrate in environments of different biophysical properties and the periodic polarity loss for the purpose of corrections to the migration route.

Publications

• Bleb Expansion in Migrating Cells Depends on Supply of Membrane from Cell Surface Invaginations. Developmental Cell, 43(5), 577-587.e5.
  Goudarzi, Mohammad; Tarbashevich, Katsiaryna; Mildner, Karina; Begemann, Isabell; Garcia, Jamie; Paksa, Azadeh; Reichman-Fried, Michal; Mahabaleshwar, Harsha; Blaser, Heiko; Hartwig, Johannes; Zeuschner, Dagmar; Galic, Milos; Bagnat, Michel; Betz, Timo & Raz, Erez
  
• Polarized actin and VE-cadherin dynamics regulate junctional remodelling and cell migration during sprouting angiogenesis. Nature Communications, 8(1).
  Cao, Jiahui; Ehling, Manuel; März, Sigrid; Seebach, Jochen; Tarbashevich, Katsiaryna; Sixta, Tomas; Pitulescu, Mara E.; Werner, Ann-Cathrin; Flach, Boris; Montanez, Eloi; Raz, Erez; Adams, Ralf H. & Schnittler, Hans
  
• Rapid progression through the cell cycle ensures efficient migration of primordial germ cells – The role of Hsp90. Developmental Biology, 436(2), 84-93.
  Pfeiffer, Jana; Tarbashevich, Katsiaryna; Bandemer, Jan; Palm, Thomas & Raz, Erez
  
• Spatio-temporal regulation of concurrent developmental processes by generic signaling downstream of chemokine receptors. eLife, 7.
  Malhotra, Divyanshu; Shin, Jimann; Solnica-Krezel, Lilianna & Raz, Erez
  
• Cellular Blebs and Membrane Invaginations Are Coupled through Membrane Tension Buffering. Biophysical Journal, 117(8), 1485-1495.
  Lavi, Ido; Goudarzi, Mohammad; Raz, Erez; Gov, Nir S.; Voituriez, Raphael & Sens, Pierre
  
• Fluid dynamics during bleb formation in migrating cells in vivo. PLOS ONE, 14(2), e0212699.
  Goudarzi, Mohammad; Boquet-Pujadas, Aleix; Olivo-Marin, Jean-Christophe & Raz, Erez
  
• Building Relationships: A Role for Innexins in Tissue Formation. Developmental Cell, 54(4), 428-430.
  Labbaf, Zahra & Raz, Erez
  
• E-cadherin focuses protrusion formation at the front of migrating cells by impeding actin flow. Nature Communications, 11(1).
  Grimaldi, Cecilia; Schumacher, Isabel; Boquet-Pujadas, Aleix; Tarbashevich, Katsiaryna; Vos, Bart Eduard; Bandemer, Jan; Schick, Jan; Aalto, Anne; Olivo-Marin, Jean-Christophe; Betz, Timo & Raz, Erez
  
• Using migrating cells as probes to illuminate features in live embryonic tissues. Science Advances, 6(49).
  Gross-Thebing, Sargon; Truszkowski, Lukasz; Tenbrinck, Daniel; Sánchez-Iranzo, Héctor; Camelo, Carolina; Westerich, Kim J.; Singh, Amrita; Maier, Paul; Prengel, Jonas; Lange, Pia; Hüwel, Jan; Gaede, Fjedor; Sasse, Ramona; Vos, Bart E.; Betz, Timo; Matis, Maja; Prevedel, Robert; Luschnig, Stefan; Diz-Muñoz, Alba; ... & Raz, Erez
  
• Chemokine-biased robust self-organizing polarization of migrating cells in vivo. Proceedings of the National Academy of Sciences, 118(7).
  Olguin-Olguin, Adan; Aalto, Anne; Maugis, Benoît; Boquet-Pujadas, Aleix; Hoffmann, Dennis; Ermlich, Laura; Betz, Timo; Gov, Nir S.; Reichman-Fried, Michal & Raz, Erez
  
• Zebrafish Primordial Germ Cell Migration. Frontiers in Cell and Developmental Biology, 9.
  Aalto, Anne; Olguin-Olguin, Adan & Raz, Erez
  
• Blebs—Formation, Regulation, Positioning, and Role in Amoeboid Cell Migration. Frontiers in Cell and Developmental Biology, 10.
  Schick, Jan & Raz, Erez
  
• Bleb protrusions help cancer cells to cheat death. Nature, 615(7952), 402-403.
  Reichman-Fried, Michal & Raz, Erez
  
• Primordial germ cells adjust their protrusion type while migrating in different tissue contexts in vivo. Development, 150(2).
  Truszkowski, Lukasz; Batur, Dilek; Long, Hongyan; Tarbashevich, Katsiaryna; Vos, Bart E.; Trappmann, Britta & Raz, Erez