Prinzipien und Mechanismen der Erkennung des X Chromosoms während der Dosiskompensation von Drosophila
Zusammenfassung der Projektergebnisse
The process of X chromosome dosage compensation in Drosophila involves doubling the transcription from the single X in male flies to match the combined output of the two X chromosomes in female flies. Transcription is activated by the ‘Dosage Compensation Complex’ (DCC), an assembly of unknown structure containing five proteins and non-coding roX RNA. The transcriptional tuning must be restricted to the X, or male viability is seriously compromised. The principles that allow the DCC to discriminate chromosomes and to interact with the X with exquisite exclusivity are still elusive. Encouraged by our recent description of a first X-specific DNA signature we devised an experimental strategy to identify X-chromosomal targeting elements monitoring the selective interactions of the DCC in vitro. A central aspect of our proposal is to assemble the entire Drosophila genome into complex, physiological chromatin, the natural substrate for DCC interactions. Deciphering the principles of X chromosome identity will end the quest for the mysterious X chromosomal determinant that inspired the field for decades, and will uncover new and general rules through which complex DNA signatures are interpreted and reveal the nature of the co-operative interactions that endow targeting systems with robustness.
Projektbezogene Publikationen (Auswahl)
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Factor cooperation for chromosome discrimination inDrosophila. Nucleic Acids Research, 47(4), 1706-1724.
Albig, Christian; Tikhonova, Evgeniya; Krause, Silke; Maksimenko, Oksana; Regnard, Catherine & Becker, Peter B.
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Genome-wide measurement of local nucleosome array regularity and spacing by nanopore sequencing. Nature Structural & Molecular Biology, 25(9), 894-901.
Baldi, Sandro; Krebs, Stefan; Blum, Helmut & Becker, Peter B.
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Genome-wide Rules of Nucleosome Phasing in Drosophila. Molecular Cell, 72(4), 661-672.e4.
Baldi, Sandro; Jain, Dhawal S.; Harpprecht, Lisa; Zabel, Angelika; Scheibe, Marion; Butter, Falk; Straub, Tobias & Becker, Peter B.
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Progressive dosage compensation during Drosophilaembryogenesis is reflected by gene arrangement. EMBO reports, 20(8).
Prayitno, Khairunnadiya; Schauer, Tamás; Regnard, Catherine & Becker, Peter B.
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Structure, dynamics and roX2-lncRNA binding of tandem double-stranded RNA binding domains dsRBD1,2 of Drosophila helicase Maleless. Nucleic Acids Research, 47(8), 4319-4333.
Ankush, Jagtap Pravin Kumar; Müller, Marisa; Masiewicz, Pawel; von Bülow; Sören; Hollmann, Nele Merret; Chen, Po-Chia; Simon, Bernd; Thomae, Andreas W.; Becker, Peter B. & Hennig, Janosch
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Cell-free genomics reveal intrinsic, cooperative and competitive determinants of chromatin interactions. Nucleic Acids Research, 49(13), 7602-7617.
Eggers, Nikolas & Becker, Peter B.
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Divergent evolution toward sex chromosome-specific gene regulation inDrosophila. Genes & Development, 35(13-14), 1055-1070.
Villa, Raffaella; Jagtap, Pravin Kumar Ankush; Thomae, Andreas W.; Campos, Sparr Aline; Forné, Ignasi; Hennig, Janosch; Straub, Tobias & Becker, Peter B.
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LncRNA RUS shapes the gene expression program towards neurogenesis. Life Science Alliance, 5(10), e202201504.
Schneider, Marius F.; Müller, Veronika; Müller, Stephan A.; Lichtenthaler, Stefan F.; Becker, Peter B. & Scheuermann, Johanna C.
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Cooperation between bHLH transcription factors and histones for DNA access. Nature, 619(7969), 385-393.
Michael, Alicia K.; Stoos, Lisa; Crosby, Priya; Eggers, Nikolas; Nie, Xinyu Y.; Makasheva, Kristina; Minnich, Martina; Healy, Kelly L.; Weiss, Joscha; Kempf, Georg; Cavadini, Simone; Kater, Lukas; Seebacher, Jan; Vecchia, Luca; Chakraborty, Deyasini; Isbel, Luke; Grand, Ralph S.; Andersch, Florian; Fribourgh, Jennifer L. ... & Thomä, Nicolas H.
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Physical interaction between MSL2 and CLAMP assures direct co-operativity and prevents competition at composite binding sites.
Eggers, Nikolas; Gkountromichos, Fotios; Krause, Silke; Campos-Sparr, Aline & Becker, Peter B.
