DNA Rekrutierung, Regulation & Funktion von SMC5/6

• Antragsteller: Dr. Markus Räschle
• Fachliche Zuordnung: Zellbiologie
• Förderung: Förderung von 2016 bis 2021
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 323666480

• Erstellungsjahr: 2021

Zusammenfassung der Projektergebnisse

In summary our study shows the potential of the egg extracts system for investigating SMC5/6 function, but clearly more work is required to optimize SMC5/6 depletion and rescue conditions. Due to the inherent synchrony of replication and the possibility to use DNA templates with defined lesions it was possible to demonstrate both replication- and DNA damage-dependent chromatin recruitment of the SMC5/6 complex. With their ability to recapitulate complex replication-coupled DNA repair reactions and the possibility to follow chromosome segregation in a biochemically tractable system, xenopus egg extracts likely will play a key role in unlocking the enigmatic functions of the SMC5/6 complex.

Projektbezogene Publikationen (Auswahl)

• Replication Coupled DNA-Protein Crosslink Repair by SPRTN and the Proteasome in Xenopus Egg Extracts. Mol Cell, 2019. 73: p. 1-15
  Larsen NB, Gao AO, Sparks JL, Gallina I, Wu RA, Mann M, Räschle M, Walter JC, Duxin JP
  
• The CMG Helicase Bypasses DNA-Protein Cross-Links to Facilitate Their Repair. Cell, 2019. 176(1-2): p. 167-181
  Sparks JL, Christol G, Gao AO, Räschle M, Larsen NB, Mann M, Duxin JP, Walter JC
  
• NSE5/6 inhibits the SMC5/6 ATPase to facilitate DNA substrate selection. (2021)
  Michael Taschner, Jérôme Basquin, Barbara Steigenberger, Ingmar Schaefer, Young-Min Soh, Claire Basquin, Esben Lorentzen, Markus Räschle, Richard A. Scheltema, Stephan Gruber