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DNA Recruitment, Regulation & Function of SMC5/6

Subject Area Cell Biology
Term from 2016 to 2021
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 323666480
 
Final Report Year 2021

Final Report Abstract

In summary our study shows the potential of the egg extracts system for investigating SMC5/6 function, but clearly more work is required to optimize SMC5/6 depletion and rescue conditions. Due to the inherent synchrony of replication and the possibility to use DNA templates with defined lesions it was possible to demonstrate both replication- and DNA damage-dependent chromatin recruitment of the SMC5/6 complex. With their ability to recapitulate complex replication-coupled DNA repair reactions and the possibility to follow chromosome segregation in a biochemically tractable system, xenopus egg extracts likely will play a key role in unlocking the enigmatic functions of the SMC5/6 complex.

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