Spatial organization and temporal dynamics of multi-replicon genomes in alpha-rhizobia (P07)

• Subject Area: Metabolism, Biochemistry and Genetics of Microorganisms
• Term: from 2017 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 269423233

This project aims at elucidating the molecular principles underlying spatio¬temporal coordination of genomic DNA replication and segregation with cell growth and division in Sinorhizobium meliloti. Specifically, we will study (i) assembly of Rgs (rhizobial growth and septation) proteins at cell division site and newest cell pole, (ii) the role of the polar scaffold proteins RgsE and PopZ in integrating cell elongation and chromosome segregation, (iii) factors involved in spatially confining the oris of the three replicons in the polar and subpolar areas, (iv) the mechanism of RepABS partition complex formation, and (v) the role of GcrA and CcrM-mediated methylation in regulating replication initiation of the secondary replicons.

• DFG Programme: CRC/Transregios
• Subproject of: TRR 174: Spatiotemporal dynamics of bacterial cells
• Applicant Institution: Philipps-Universität Marburg
• Project Head: Professorin Dr. Anke Becker