EXC 168:  Zentrum für Regenerative Therapien Dresden (CRTD)

• Fachliche Zuordnung: Medizin ; Neurowissenschaften
• Förderung: Förderung von 2006 bis 2019
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 32725986

• Erstellungsjahr: 2021

Zusammenfassung der Projektergebnisse

Das Zentrum für Regenerative Therapien Dresden (CRTD) hat führende Wissenschaftler aus den Bereichen Entwicklungs-, Regenerations- und Stammzellbiologie vereint, um die Forschung zu Geweberegeneration und Zellersatz voranzutreiben. Das Prinzip des CRTD, die Entdeckung aus erfolgreicher Regeneration und Entwicklung, basiert auf außergewöhnlicher Forschung an Stammzellen und mit Modellorganismen, die ihre Fähigkeit zur Regeneration von Geweben nach einer Schädigung bewahren. Ein effektives Merkmal des CRTD ist die wissenschaftliche Vielfalt der Modelle, Organe, Krankheiten und Ansätze, die durch die gemeinsamen Ziele und die Nähe von Wissenschaftlern und Klinikern synergetisch genutzt werden. Mit der Förderung konnte ein Netzwerk aufgebaut werden, das die Grundlagenforschung der bestehenden Technische Universität Dresden (TU Dresden) Forschungsinstitute mit klinischen Instituten und mit außeruniversitären Instituten eng verbindet. Der Einsatz von Seed Grants und Gerok- Positionen schuf den Rahmen für die interdisziplinäre Verzahnung. Der Ausbau und das gezielte Schließen von Lücken im Großgerätebereich in den Technologieplattformen und im Service- und Gerätepool hat dem Campus einen Vorteil verschafft. Innerhalb des Clusters konnten neue Ansätze etabliert werden, die dann auf den Campus ausgedehnt wurden, wie z.B. Tenure Track Optionen oder flexible Kinderbetreuung. Die Grundlagenforschung im Bereich regenerative Modellorganismen des CRTD konnte international renommierte Forscher der Zebrafisch- und Salamanderforschung sowie Experten für Säugetiermodelle integrieren. Die Forscher haben bahnbrechende Entdeckungen gemacht, die neue Forschungswege eröffnen und die Dresdener Forschung prägt. Die Zelltherapie ist ein weiteres zentrales Forschungsgebiet. Um dieses strategische Ziel des CRTD zu erreichen, wurde in Dresden die Inseltransplantation der Bauchspeicheldrüse erstmalig in Deutschland etabliert. Diese Fortschritte bieten auch Möglichkeiten für die Transplantation von hESC- und hiPSC-basierten Beta-Zellen. Zell-, organoid- und in vivo basierte Methoden zur Pathogenese und Identifizierung von Wirkstoffzielen wurden durch exzellente Dresdner Entdeckungen und Rekrutierung entwickelt. Eine Grundlage für die Entdeckung pathogenetischer Mechanismen und des Wirkstoffscreenings wurde durch in vivo Zebrafisch-Drug-Screening geschaffen. Die Entdeckungen, die für hämatopoetische Stamm- und Vorläuferzellen (HSPC) und die pankreatische Beta-Zell-Expansion relevant sind, ermöglichen eine erfolgreiche iPSC/ESC/ gene editing Facility. Es gab auch therapeutisch relevante zellbasierte Entdeckungen bei Kindern mit immunologischen Erkrankungen, darunter bahnbrechende Geburtskohorten für Typ-1-Diabetes und Immunität sowie technologische Entwicklungen mit Relevanz für Fische über Mäuse bis hin zum Menschen. Der Freistaat Sachsen und die TU Dresden haben das CRTD mit einer jährlichen Förderung von derzeit 9,9 Mio. € dauerhaft übernommen. Das CRTD ist als eines von drei Instituten innerhalb des Center for Molecular and Cell Bioengineering (CMCB) organisiert, einer fakultätsähnlichen Verwaltungsplattform für CRTD, B CUBE und BIOTEC. Das CRTD wird somit weiterhin seine wissenschaftliche Mission verfolgen und seine Arbeit im Bereich der Regenerativen Biologie - und Gewebeforschung fortsetzen können.

Link zum Abschlussbericht

https://doi.org/10.2314/KXP:1746737352

Projektbezogene Publikationen (Auswahl)

• (2006). Neural stem cells and neurogenesis in the adult zebrafish brain: origin, proliferation dynamics, migration and cell fate. Developmental Biology, 295(1):263-277
  Grandel, H., Kaslin, J., Ganz, J., Wenzel, I., and Brand, M.
  
• (2007). HIV-1 proviral DNA excision using an evolved recombinase. Science 316, 1912-1915
  Sarkar, I., Hauber, I., Hauber, J., and Buchholz, F.
  
• Foxp3 occupancy and regulation of key target genes during T-cell stimulation. Nature. 2007 Feb 22;445(7130):931-5
  Marson A, Kretschmer K, Frampton GM, Jacobsen ES, Polansky JK, MacIsaac KD, Levine SS, Fraenkel E, von Boehmer H, Young RA
  
• (2009). A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity. Cell Stem Cell 4, 403-415
  Ding, L., Paszkowski-Rogacz, M., Nitzsche, A., Slabicki, M.M., Heninger, A.K., de Vries, I., Kittler, R., Junqueira, M., Shevchenko, A., Schulz, H., et al.
  
• (2009). Cells keep a memory of their tissue origin during axolotl limb regeneration. Nature 460, 60-65
  Kragl, M., Knapp, D., Nacu, E., Khattak, S., Maden, M., Epperlein, H.H., and Tanaka, E.M.
  
• (2009). FGF8 morphogen gradient is formed by a source-sink mechanism with freely-diffusing molecules. Nature 461(7263): 533-536
  Yu, S.R., Burkhardt, M., Nowak, M., Ries, J., Petrášek, Z., Scholpp, S., Schwille, P. and Brand, M.
  
• (2009). Modular scanning FCS quantifies ligand-receptor interactions in live multicellular organisms. Nature Methods 6(9):643-645
  Ries, J. , Yu, S. R., Burkhardt, M., Brand, M. and Schwille, P.
  
• (2009). Temporally-controlled Sitespecific Recombination in Zebrafish. PLoS One, 4(2):e4640
  Hans, S., Kaslin, J., Freudenreich, D., and Brand, M.
  
• (2009). Treatment of refractory acute GVHD with third-party MSC expanded in platelet lysate-containing medium. Bone Marrow Transplant 43, 245-251
  von Bonin, M., Stolzel, F., Goedecke, A., Richter, K., Wuschek, N., Holig, K., Platzbecker, U., Illmer, T., Schaich, M., Schetelig, J., et al.
  
• (2010) Heterogeneity and Fgf dependence of adult neural progenitors in the zebrafish telencephalon. Glia, 58(11):1345- 1363
  Ganz J., Kaslin J., Hochmann S., Freudenreich D., Brand M.
  
• (2010) Promoting tolerance to proteolipid protein-induced experimental autoimmune encephalomyelitis through targeting dendritic cells. Proc Natl Acad Sci USA. 107(40):17280-5
  Stern JN, Keskin DB, Kato Z, Waldner H, Schallenberg S, Anderson A, von Boehmer H, Kretschmer K, Strominger JL
  
• (2010). A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. PLoS biology, 8(6), e1000408
  Słabicki, M., Theis, M., Krastev, D. B., Samsonov, S., Mundwiller, E., Junqueira, M., Paszkowski-Rogacz, M., Teyra, J., Heninger, A. K., Poser, I., Prieur, F., Truchetto, J., Confavreux, C., Marelli, C., Durr, A., Camdessanche, J. P., Brice, A., Shevchenko, A., Pisabarro, M. T., Stevanin, G., … Buchholz, F.
  
• (2010). Cancellous bone allograft seeded with human mesenchymal stromal cells: a potential good manufacturing practice-grade tool for the regeneration of bone defects. Cytotherapy 12, 658-668
  Stiehler, M., Seib, F.P., Rauh, J., Goedecke, A., Werner, C., Bornhauser, M., Gunther, K.P., and Bernstein, P.
  
• (2010). Dually inducible TetON systems for tissue-specific conditional gene expression in zebrafish. Proc Natl Acad Sci U S A 107, 19933-19938
  Knopf, F., Schnabel, K., Haase, C., Pfeifer, K., Anastassiadis, K., and Weidinger, G.
  
• Identification of an immediate Foxp3(- ) precursor to Foxp3(+) regulatory T cells in peripheral lymphoid organs of nonmanipulated mice. J Exp Med. 2010 Jul 5;207(7):1393-407
  Schallenberg S, Tsai PY, Riewaldt J, Kretschmer K
  
• (2011) Overexpression of Cdk4 and cyclinD1 triggers a greater expansion of neural stem cells in the adult mouse brain. J Exp Med, 208:937-48
  Artegiani B, Lindemann D and Calegari F
  
• (2011). A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly. Nat Cell Biol 13, 809-818
  Krastev, D.B., Slabicki, M., Paszkowski-Rogacz, M., Hubner, N.C., Junqueira, M., Shevchenko, A., Mann, M., Neugebauer, K.M., and Buchholz, F.
  
• (2011). Interpretation of the FGF8 morphogen gradient is regulated by endocytic trafficking. Nat Cell Biol 13, 153-158
  Nowak, M., Machate, A., Yu, S.R., Gupta, M., and Brand, M.
  
• (2011). Neurogenic radial glia-type progenitors contribute to regeneration of the adult zebrafish brain. Development 138, 4831-4841
  Kroehne, V., Freudenreich, D., Hans, S., Kaslin, J., Brand, M.
  
• 2011. Bone Regenerates via Dedifferentiation of Osteoblasts in the Zebrafish Fin. Dev Cell 20, 713-724
  Knopf, F., Hammond, C., Chekuru, A., Kurth, T., Hans, S., Weber, C.W., Mahatma, G., Fisher, S., Brand, M., Schulte-Merker, S., Weidinger, G.
  
• Osteoporosis: now and the future. Lancet 2011;377:1276-87
  Rachner TD, Khosla S, Hofbauer LC
  
• Prophylactic transfer of BCR-ABL-, PR1-, and WT1- reactive donor T cells after T cell-depleted allogeneic hematopoietic cell transplantation in patients with chronic myeloid leukemia. Blood. 2011 Jun 30;117(26):7174-84
  Bornhäuser M, Thiede C, Platzbecker U, Kiani A, Oelschlaegel U, Babatz J, Lehmann D, Hölig K, Radke J, Tuve S, Wermke M, Wehner R, Jähnisch H, Bachmann MP, Rieber EP, Schetelig J, Ehninger G, Schmitz M
  
• (2012). Acute inflammation initiates the regenerative response in the adult zebrafish brain. Science 338, 1353-1356
  Kyritsis, N., Kizil, C., Zocher, S., Kroehne, V., Kaslin, J., Freudenreich, D., Iltzsche, A., and Brand, M.
  
• (2012). Combined RNAi and localization for functionally dissecting long noncoding RNAs. Nat Methods 9, 360-362
  Chakraborty, D., Kappei, D., Theis, M., Nitzsche, A., Ding, L., Paszkowski-Rogacz, M., Surendranath, V., Berger, N., Schulz, H., Saar, K., et al.
  
• (2012). Reconstitution of the central and peripheral nervous system during salamander tail regeneration. Proc Natl Acad Sci U S A 109, E2258- 2266
  Mchedlishvili, L., Mazurov, V., Grassme, K.S., Goehler, K., Robl, B., Tazaki, A., Roensch, K., Duemmler, A., and Tanaka, E.M.
  
• (2012). Regenerative neurogenesis from neural progenitor cells requires injury-induced expression of Gata3. Dev Cell, 23(6):1230-1237
  Kizil, C., Kyritsis, N., Dudczig, S., Kroehne, V., Freudenreich, D., Kaslin, J. and Brand, M.
  
• Age-related islet autoantibody incidence in offspring of patients with type 1 diabetes. Diabetologia. 2012 Jul;55(7):1937-43
  Ziegler AG, Bonifacio E; BABYDIAB-BABYDIET Study Group
  
• Reduced-intensity conditioning versus standard conditioning before allogeneic haemopoietic cell transplantation in patients with acute myeloid leukaemia in first complete remission: a prospective, open-label randomised phase 3 trial. Lancet Oncol. 2012 Oct;13(10):1035-44
  Bornhäuser M, Kienast J, Trenschel R, Burchert A, Hegenbart U, Stadler M, Baurmann H, Schäfer-Eckart K, Holler E, Kröger N, Schmid C, Einsele H, Kiehl MG, Hiddemann W, Schwerdtfeger R, Buchholz S, Dreger P, Neubauer A, Berdel WE, Ehninger G, Beelen DW, Schetelig J, Stelljes M
  
• (2013) Regulation of cerebral cortex size and folding by expansion of basal progenitors. EMBO J, 32:1817-28
  Nonaka-Kinoshita M, Reillo I, Artegiani B, Martinez M, Nelson M, Borrell V and Calegari F
  
• (2013) Sclerostin antibody treatment improves bone mass, bone strength, and bone defect regeneration in rats with type 2 diabetes mellitus. J Bone Miner Res. 28:627-38
  Hamann C, Rauner M, Höhna Y, Bernhardt R, Mettelsiefen J, Goettsch C, Günther KP, Stolina M, Han CY, Asuncion FJ, Ominsky MS, Hofbauer LC
  
• (2013) Transcriptome sequencing during mouse brain development identifies long non-coding RNAs functionally involved in neurogenic commitment. EMBO J, 32:3145-60
  Aprea J, Prenninger S, Dori M, Sebastian Monasor L, Wessendorf E, Zocher S, Massalini S, Ghosh T, Alexopoulou D, Lesche M, Dahl A, Groszer M, Hiller M and Calegari F
  
• (2013). Assessing computational methods for transcription factor target gene identification based on ChIP-seq data. PLoS Comput Biol 9, e1003342
  Sikora-Wohlfeld, W., Ackermann, M., Christodoulou, E.G., Singaravelu, K., and Beyer, A.
  
• (2013). CD133 is a modifier of hematopoietic progenitor frequencies but is dispensable for the maintenance of mouse hematopoietic stem cells. Proc Natl Acad Sci USA. 110(14):5582-7
  Arndt K, Grinenko T, Mende N, Reichert D, Portz M, Ripich T, Carmeliet P, Corbeil D, Waskow C
  
• (2013). Characterization of light lesion paradigms and optical coherence tomography as tools to study adult retina regeneration in zebrafish. PLoS One 8, e80483
  Weber, A., Hochmann, S., Cimalla, P., Gartner, M., Kuscha, V., Hans, S., Geffarth, M., Kaslin, J., Koch, E., and Brand, M.
  
• (2013). Defined polymer-peptide conjugates to form cell-instructive starPEG-heparin matrices in situ. Adv Mater 25, 2606-2610
  Tsurkan, M.V., Chwalek, K., Prokoph, S., Zieris, A., Levental, K.R., Freudenberg, U., and Werner, C.
  
• (2013). Development and specification of cerebellar stem and progenitor cells in zebrafish: from embryo to adult. Neural Dev 8, 9
  Kaslin, J., Kroehne, V., Benato, F., Argenton, F., and Brand, M.
  
• (2013). Emergence of Individuality in Genetically Identical Mice. Science 340, 6133: 756-9
  Freund, J., A. M. Brandmaier, L. Lewejohann, I. Kirste, M. Kritzler, A. Kruger, N. Sachser, U. Lindenberger, and G. Kempermann
  
• (2013). Progressive specification rather than intercalation of segments during limb regeneration. Science 342, 1375-1379
  Roensch, K., Tazaki, A., Chara, O., and Tanaka, E.M.
  
• (2013). Seroconversion to multiple islet autoantibodies and risk of progression to diabetes in children. JAMA 309, 2473-2479
  Ziegler, A.G., Rewers, M., Simell, O., Simell, T., Lempainen, J., Steck, A., Winkler, C., Ilonen, J., Veijola, R., Knip, M., Bonifacio, E., and Eisenbarth, G.S.
  
• (2013). Transplantation of human islets without immunosuppression. Proc Natl Acad Sci USA 110, 19054-19058
  Ludwig, B., Reichel, A., Steffen, A., Zimerman, B., Schally, A. V., Block, N. L., Colton, C. K., Ludwig, S., Kersting, S., Bonifacio, E., Solimena, M., Gendler, Z., Rotem, A., Barkai, U., and Bornstein, S. R.
  
• (2013). Transposon mediated BAC transgenesis via pronuclear injection of mouse zygotes. Genesis 51, 135-141
  Rostovskaya, M., Naumann, R., Fu, J., Obst, M., Mueller, D., Stewart, A.F., and Anastassiadis, K.
  
• (2014) Clonal expansion capacity defines two consecutive developmental stages of long-term hematopoietic stem cells. J Exp Med. 2014 Feb 10;211(2):209-15
  Grinenko T, Arndt K, Portz M, Mende N, Günther M, Cosgun KN, Alexopoulou D, Lakshmanaperumal N, Henry I, Dahl A, Waskow C
  
• (2014) CXCR4 blockade and sphingosine-1-phosphate activation facilitate engraftment of haematopoietic stem and progenitor cells in a non-myeloablative transplant model. Br J Haematol. 164(3):409-13
  Shayegi N, Meyer C, Lambert K, Ehninger G, Brand M, Bornhäuser M
  
• (2014) Kit regulates HSC engraftment across the humanmouse species barrier. Cell Stem Cell 7;15(2):227-38
  Cosgun KN, Rahmig S, Mende N, Reinke S, Hauber I, Schäfer C, Petzold A, Weisbach H, Heidkamp G, Purbojo A, Cesnjevar R, Platz A, Bornhäuser M, Schmitz M, Dudziak D, Hauber J, Kirberg J, Waskow C
  
• (2014) Letermovir for cytomegalovirus prophylaxis in hematopoietic-cell transplantation. N Engl J Med. 8;370(19):1781-9
  Chemaly RF, Ullmann AJ, Stoelben S, Richard MP, Bornhäuser M, Groth C, Einsele H, Silverman M, Mullane KM, Brown J, Nowak H, Kölling K, Stobernack HP, Lischka P, Zimmermann H, Rübsamen-Schaeff H, Champlin RE, Ehninger G; AIC246 Study Team
  
• (2014). Fundamental differences in dedifferentiation and stem cell recruitment during skeletal muscle regeneration in two salamander species. Cell Stem Cell 14, 174-187
  Sandoval-Guzman, T., Wang, H., Khattak, S., Schuez, M., Roensch, K., Nacu, E., Tazaki, A., Joven, A., Tanaka, E.M., and Simon, A.
  
• (2014). Glycosaminoglycanbased hydrogels to modulate heterocellular communication in in vitro angiogenesis models. Scientific Reports 4
  Chwalek, K., Tsurkan, M.V., Freudenberg, U., and Werner, C.
  
• (2014). Neuroinflammation and central nervous system regeneration in vertebrates. Trends in Cell Biology
  Kyritsis, N., Kizil, C. and Brand, M.
  
• (2014). Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biology. Nature protocols, 9(12), 2809–2822
  Marciniak, A., Cohrs, C. M., Tsata, V., Chouinard, J. A., Selck, C., Stertmann, J., Reichelt, S., Rose, T., Ehehalt, F., Weitz, J., Solimena, M., Slak Rupnik, M. and Speier, S.
  
• 2014. Calcineurin regulates coordinated outgrowth of zebrafish regenerating fins. Dev Cell 28, 573-587
  Kujawski, S., Lin, W., Kitte, F., Bormel, M., Fuchs, S., Arulmozhivarman, G., Vogt, S., Theil, D., Zhang, Y., Antos, C.L.
  
• (2015) CCND1-CDK4-mediated cell cycle progression provides a competitive advantage for human hematopoietic stem cells in vivo. J Exp Med. ;212(8):1171-83
  Mende N, Kuchen EE, Lesche M, Grinenko T, Kokkaliaris KD, Hanenberg H, Lindemann D, Dahl A, Platz A, Höfer T, Calegari F, Waskow C
  
• (2015). Adoptive transfer of allogeneic regulatory T cells into patients with chronic graft-versus-host disease. Cytotherapy 17, 473-486
  Theil, A., Tuve, S., Oelschlagel, U., Maiwald, A., Dohler, D., Ossmann, D., Zenkel, A., Wilhelm, C., Middeke, J.M., Shayegi, N., et al.
  
• (2015). Distinct roles of beta-cell mass and function during type 1 diabetes onset and remission. Diabetes 64, 2148-2160
  Chmelova, H., Cohrs, C.M., Chouinard, J.A., Petzold, C., Kuhn, M., Chen, C., Roeder, I., Kretschmer, K., and Speier, S.
  
• (2015). Effects of high-dose oral insulin on immune responses in children at high risk for type 1 diabetes: the Pre-POINT randomized clinical trial. JAMA 313, 1541
  Bonifacio E, Ziegler AG, Klingensmith G, Schober E, Bingley PJ, Rottenkolber M, Theil A, Eugster A, Puff R, Peplow C, Buettner F, Lange K, Hasford J, Achenbach P, and the Pre- POINT Study Group
  
• (2015). Human-specific gene ARHGAP11B promotes basal progenitor amplification and neocortex expansion. Science 347, 1465-1470
  Florio, M., Albert, M., Taverna, E., Namba, T., Brandl, H., Lewitus, E., Haffner, C., Sykes, A., Wong, F.K., Peters, J., et al.
  
• (2015). No Evidence for Cardiomyocyte Number Expansion in Preadolescent Mice. Cell 163, 1026-1036
  Alkass, K., Panula, J., Westman, M., Wu, T.D., Guerquin-Kern, J.L., and Bergmann, O.
  
• (2015). Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration. Elife 4, e10230
  Rodrigo Albors, A., Tazaki, A., Rost, F., Nowoshilow, S., Chara, O., and Tanaka, E.M.
  
• (2015). RNAi profiling of primary human AML cells identifies ROCK1 as a therapeutic target and nominates fasudil as an antileukemic drug. Blood 125, 3760-3768
  Wermke, M., Camgoz, A., Paszkowski-Rogacz, M., Thieme, S., von Bonin, M., Dahl, A., Platzbecker, U., Theis, M., Ehninger, G., Brenner, S., et al.
  
• (2015). Tox: a multifunctional transcription factor and novel regulator of mammalian corticogenesis. EMBO J 34, 896-910
  Artegiani, B., de Jesus Domingues, A.M., Bragado Alonso, S., Brandl, E., Massalini, S., Dahl, A., and Calegari, F.
  
• Structural and functional insights into sclerostin-glycosaminoglycan interactions in bone. Biomaterials. 2015 Jul 15;67:335-345
  Salbach-Hirsch J, Samsonov SA, Hintze V, Picke AK, Rauner M, Gehrcke JP, Moeller S, Schnabelrauch M, Scharnweber D, Pisabarro MT, Hofbauer LC
  
• (2016). Aldehyde dehydrogenase activity is necessary for beta cell development and functionality in mice. Diabetologia 59, 139-150
  Anastasiou, V., Ninou, E., Alexopoulou, D., Stertmann, J., Muller, A., Dahl, A., Solimena, M., Speier, S., Serafimidis, I., and Gavalas, A.
  
• (2016). FGF8 and SHH substitute for anterior-posterior tissue interactions to induce limb regeneration. Nature 533, 407-410
  Nacu, E., Gromberg, E., Oliveira, C.R., Drechsel, D., and Tanaka, E.M.
  
• (2016). Identification of proliferative and mature β-cells in the islets of Langerhans. Nature 535(7612):430-4
  Bader E, Migliorini A, Gegg M, … , Chmelova H, Chouinard JA, Oskolkov N, Korsgren O, Speier S, Lickert H
  
• (2016). Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools. Dev Cell 39, 411-423
  Currie, J.D., Kawaguchi, A., Traspas, R.M., Schuez, M., Chara, O., and Tanaka, E.M.
  
• (2016). MARCKS-like protein is an initiating molecule in axolotl appendage regeneration. Nature 531, 237-240
  Sugiura, T., Wang, H., Barsacchi, R., Simon, A., and Tanaka, E.M.
  
• (2016). Mesenchymal Stromal Cells for Treatment of Acute Steroid-Refractory Graft Versus Host Disease: Clinical Responses and Long-Term Outcome. Stem Cells 34, 357-366
  von Dalowski, F., Kramer, M., Wermke, M., Wehner, R., Rollig, C., Alakel, N., Stolzel, F., Parmentier, S., Sockel, K., Krech, M., et al.
  
• (2016). Retinal Organoids from Pluripotent Stem Cells Efficiently Recapitulate Retinogenesis. Stem Cell Reports 6, 4: 525-38
  Volkner, M., M. Zschatzsch, M. Rostovskaya, R. W. Overall, V. Busskamp, K. Anastassiadis, and M. O. Karl
  
• Directed evolution of a recombinase that excises the provirus of most HIV-1 primary isolates with high specificity. Nat Biotechnol. 2016 Apr;34(4):401-9
  Karpinski J, Hauber I, Chemnitz J, Schäfer C, Paszkowski-Rogacz M, Chakraborty D, Beschorner N, Hofmann-Sieber H, Lange UC, Grundhoff A, Hackmann K, Schrock E, Abi- Ghanem J, Pisabarro MT, Surendranath V, Schambach A, Lindner C, van Lunzen J, Hauber J, Buchholz F
  
• Increased EPO Levels Are Associated with Bone Loss in Mice Lacking PHD2 in EPO-Producing Cells. J Bone Miner Res. 31(10):1877-1887, 2016
  Rauner M, Franke K, Murray M, Singh RP, Hiram-Bab S, Platzbecker U, Gassmann M, Socolovsky M, Neumann D, Gabet Y, Chavakis T, Hofbauer LC, Wielockx B
  
• Retinal Transplantation of Photoreceptors Results in Donor-Host Cytoplasmic Exchange. (2016). Nat Commun 7: 13028
  Santos-Ferreira, T., S. Llonch, O. Borsch, K. Postel, J. Haas, and M. Ader
  
• (2017) Immune suppressive and bone inhibitory effects of prednisolone in growing and regenerating zebrafish tissues. J Bone Miner Res. 32:2476-88
  Geurtzen K, Vernet A, Freidin A, Rauner M, Hofbauer LC, Schneider JE, Brand M, Knopf F
  
• (2017). A divergent population of autoantigen-responsive CD4+ T cells in infants prior to beta cell autoimmunity. Sci Transl Med 9
  Heninger AK, Eugster A, Kuehn D, Buettner F, Kuhn M, Lindner A, Dietz S, Jergens S, Wilhelm C, Beyerlein A, Ziegler AG, Bonifacio E
  
• (2017). A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags. Sci Rep 7, 23
  Muller, A., Neukam, M., Ivanova, A., Sonmez, A., Munster, C., Kretschmar, S., Kalaidzidis, Y., Kurth, T., Verbavatz, J.M., and Solimena, M.
  
• (2017). Clonal fate-mapping quantifies the number of haematopoietic stem cells that arise during development. Nature Cell Biology, 19(1):17-27
  Henninger, J., Santoso, B., Hans, S., Durand, E., Moore, J., Mosimann, C., Brand, M., Traver, D., and Zon, L.
  
• (2017). Combined influence of biophysical and biochemical cues on maintenance and proliferation of hematopoietic stem cells. Biomaterials 138, 108-117
  Gvaramia, D., Muller, E., Muller, K., Atallah, P., Tsurkan, M., Freudenberg, U., Bornhauser, M., and Werner, C.
  
• (2017). Different developmental histories of beta-cells generate functional and proliferative heterogeneity during islet growth. Nat Commun 8, 664
  Singh, S.P., Janjuha, S., Hartmann, T., Kayisoglu, O., Konantz, J., Birke, S., Murawala, P., Alfar, E.A., Murata, K., Eugster, A., Tsuji N, Morrissey ER, Brand M, Ninov N.
  
• (2017). Distinct roles of neuroepithelial-like and radial glia-like progenitor cells in cerebellar regeneration. Development, 144, 1462-1471
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