EXC 168:  Regenerative Therapies - From Cells to Tissues to Therapies: Engineering the Cellular Basis of Regeneration

• Subject Area: Medicine ; Neurosciences
• Term: from 2006 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 32725986

• Final Report Year: 2021

Final Report Abstract

The Center for Regenerative Therapies Dresden (CRTD) has united leading scientists in the fields of developmental, regenerative, and stem cell biology to advance research into tissue regeneration and cell replacement. The principle of the CRTD, discovery from successful regeneration and development, is based on exceptional research with stem cells and model organisms that retain their ability to regenerate tissues after damage. An effective feature of the CRTD is scientific diversity in the models, organs, diseases, and approaches that are synergised by the shared objectives and close proximity of scientists and clinicians. The funding enabled the establishment of a network that closely links the basic research of the existing Technische Universität Dresden (TU Dresden) research institutes with clinical institutes and with non-university institutes. The use of Seed Grants and Gerok positions created the framework for interdisciplinary intermeshing. The expansion and targeted closing of gaps in large-scale equipment in the Technology Platforms and the Service and Device Pool has given the campus an advantage. Within the cluster, new approaches could be established, which were then expanded to benefit the campus at large, such as tenure track options or flexible child care. CRTD’s regenerative model organism research base has integrated leaders of international repute in zebrafish and salamander research, and experts in mammalian animal models. Investigators have made seminal discoveries that opened new research routes and provided unique Dresden traits. Cell therapy is a further key research area. Fulfilling a CRTD strategic objective, pancreatic islet transplantation was established in Dresden as a ‘first’ in Germany. These advances also provide opportunities for the transplantation of hESC- or hiPSC-derived beta-cells. Cell-, organoid-, and in vivo-based methods for pathogenesis and drug target identification have been developed through excellent Dresden discoveries and recruitment. A basis for the discovery of pathogenetic mechanisms and drug screening was established via in vivo zebrafish drug screening, providing discoveries relevant to haematopoietic stem and progenitor cells (HSPC) and pancreatic beta-cell expansion, and a successful iPSC/ESC/gene editing facility. There have also been therapeutically relevant cell-based discoveries in children with immunological diseases, including pioneering birth cohorts for type 1 diabetes and immunity, and technological developments relevant from fish to mouse to humans. The Free State of Saxony and the TU Dresden will permanently continue the CRTD with annual funding of currently 9.9 Mio €. The CRTD is embedded as one of three institutes within the Center for Molecular and Cell Bioengineering (CMCB), a faculty-like institution of the TU Dresden and an administrative platform for CRTD, B CUBE and BIOTEC. The CRTD will continue to pursue its own scientific mission and provide regenerative biology research.

Link to the final report

https://doi.org/10.2314/KXP:1746737352

Publications

• (2006). Neural stem cells and neurogenesis in the adult zebrafish brain: origin, proliferation dynamics, migration and cell fate. Developmental Biology, 295(1):263-277
  Grandel, H., Kaslin, J., Ganz, J., Wenzel, I., and Brand, M.
  
• (2007). HIV-1 proviral DNA excision using an evolved recombinase. Science 316, 1912-1915
  Sarkar, I., Hauber, I., Hauber, J., and Buchholz, F.
  
• Foxp3 occupancy and regulation of key target genes during T-cell stimulation. Nature. 2007 Feb 22;445(7130):931-5
  Marson A, Kretschmer K, Frampton GM, Jacobsen ES, Polansky JK, MacIsaac KD, Levine SS, Fraenkel E, von Boehmer H, Young RA
  
• (2009). A genome-scale RNAi screen for Oct4 modulators defines a role of the Paf1 complex for embryonic stem cell identity. Cell Stem Cell 4, 403-415
  Ding, L., Paszkowski-Rogacz, M., Nitzsche, A., Slabicki, M.M., Heninger, A.K., de Vries, I., Kittler, R., Junqueira, M., Shevchenko, A., Schulz, H., et al.
  
• (2009). Cells keep a memory of their tissue origin during axolotl limb regeneration. Nature 460, 60-65
  Kragl, M., Knapp, D., Nacu, E., Khattak, S., Maden, M., Epperlein, H.H., and Tanaka, E.M.
  
• (2009). FGF8 morphogen gradient is formed by a source-sink mechanism with freely-diffusing molecules. Nature 461(7263): 533-536
  Yu, S.R., Burkhardt, M., Nowak, M., Ries, J., Petrášek, Z., Scholpp, S., Schwille, P. and Brand, M.
  
• (2009). Modular scanning FCS quantifies ligand-receptor interactions in live multicellular organisms. Nature Methods 6(9):643-645
  Ries, J. , Yu, S. R., Burkhardt, M., Brand, M. and Schwille, P.
  
• (2009). Temporally-controlled Sitespecific Recombination in Zebrafish. PLoS One, 4(2):e4640
  Hans, S., Kaslin, J., Freudenreich, D., and Brand, M.
  
• (2009). Treatment of refractory acute GVHD with third-party MSC expanded in platelet lysate-containing medium. Bone Marrow Transplant 43, 245-251
  von Bonin, M., Stolzel, F., Goedecke, A., Richter, K., Wuschek, N., Holig, K., Platzbecker, U., Illmer, T., Schaich, M., Schetelig, J., et al.
  
• (2010) Heterogeneity and Fgf dependence of adult neural progenitors in the zebrafish telencephalon. Glia, 58(11):1345- 1363
  Ganz J., Kaslin J., Hochmann S., Freudenreich D., Brand M.
  
• (2010) Promoting tolerance to proteolipid protein-induced experimental autoimmune encephalomyelitis through targeting dendritic cells. Proc Natl Acad Sci USA. 107(40):17280-5
  Stern JN, Keskin DB, Kato Z, Waldner H, Schallenberg S, Anderson A, von Boehmer H, Kretschmer K, Strominger JL
  
• (2010). A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia. PLoS biology, 8(6), e1000408
  Słabicki, M., Theis, M., Krastev, D. B., Samsonov, S., Mundwiller, E., Junqueira, M., Paszkowski-Rogacz, M., Teyra, J., Heninger, A. K., Poser, I., Prieur, F., Truchetto, J., Confavreux, C., Marelli, C., Durr, A., Camdessanche, J. P., Brice, A., Shevchenko, A., Pisabarro, M. T., Stevanin, G., … Buchholz, F.
  
• (2010). Cancellous bone allograft seeded with human mesenchymal stromal cells: a potential good manufacturing practice-grade tool for the regeneration of bone defects. Cytotherapy 12, 658-668
  Stiehler, M., Seib, F.P., Rauh, J., Goedecke, A., Werner, C., Bornhauser, M., Gunther, K.P., and Bernstein, P.
  
• (2010). Dually inducible TetON systems for tissue-specific conditional gene expression in zebrafish. Proc Natl Acad Sci U S A 107, 19933-19938
  Knopf, F., Schnabel, K., Haase, C., Pfeifer, K., Anastassiadis, K., and Weidinger, G.
  
• Identification of an immediate Foxp3(- ) precursor to Foxp3(+) regulatory T cells in peripheral lymphoid organs of nonmanipulated mice. J Exp Med. 2010 Jul 5;207(7):1393-407
  Schallenberg S, Tsai PY, Riewaldt J, Kretschmer K
  
• (2011) Overexpression of Cdk4 and cyclinD1 triggers a greater expansion of neural stem cells in the adult mouse brain. J Exp Med, 208:937-48
  Artegiani B, Lindemann D and Calegari F
  
• (2011). A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly. Nat Cell Biol 13, 809-818
  Krastev, D.B., Slabicki, M., Paszkowski-Rogacz, M., Hubner, N.C., Junqueira, M., Shevchenko, A., Mann, M., Neugebauer, K.M., and Buchholz, F.
  
• (2011). Interpretation of the FGF8 morphogen gradient is regulated by endocytic trafficking. Nat Cell Biol 13, 153-158
  Nowak, M., Machate, A., Yu, S.R., Gupta, M., and Brand, M.
  
• (2011). Neurogenic radial glia-type progenitors contribute to regeneration of the adult zebrafish brain. Development 138, 4831-4841
  Kroehne, V., Freudenreich, D., Hans, S., Kaslin, J., Brand, M.
  
• 2011. Bone Regenerates via Dedifferentiation of Osteoblasts in the Zebrafish Fin. Dev Cell 20, 713-724
  Knopf, F., Hammond, C., Chekuru, A., Kurth, T., Hans, S., Weber, C.W., Mahatma, G., Fisher, S., Brand, M., Schulte-Merker, S., Weidinger, G.
  
• Osteoporosis: now and the future. Lancet 2011;377:1276-87
  Rachner TD, Khosla S, Hofbauer LC
  
• Prophylactic transfer of BCR-ABL-, PR1-, and WT1- reactive donor T cells after T cell-depleted allogeneic hematopoietic cell transplantation in patients with chronic myeloid leukemia. Blood. 2011 Jun 30;117(26):7174-84
  Bornhäuser M, Thiede C, Platzbecker U, Kiani A, Oelschlaegel U, Babatz J, Lehmann D, Hölig K, Radke J, Tuve S, Wermke M, Wehner R, Jähnisch H, Bachmann MP, Rieber EP, Schetelig J, Ehninger G, Schmitz M
  
• (2012). Acute inflammation initiates the regenerative response in the adult zebrafish brain. Science 338, 1353-1356
  Kyritsis, N., Kizil, C., Zocher, S., Kroehne, V., Kaslin, J., Freudenreich, D., Iltzsche, A., and Brand, M.
  
• (2012). Combined RNAi and localization for functionally dissecting long noncoding RNAs. Nat Methods 9, 360-362
  Chakraborty, D., Kappei, D., Theis, M., Nitzsche, A., Ding, L., Paszkowski-Rogacz, M., Surendranath, V., Berger, N., Schulz, H., Saar, K., et al.
  
• (2012). Reconstitution of the central and peripheral nervous system during salamander tail regeneration. Proc Natl Acad Sci U S A 109, E2258- 2266
  Mchedlishvili, L., Mazurov, V., Grassme, K.S., Goehler, K., Robl, B., Tazaki, A., Roensch, K., Duemmler, A., and Tanaka, E.M.
  
• (2012). Regenerative neurogenesis from neural progenitor cells requires injury-induced expression of Gata3. Dev Cell, 23(6):1230-1237
  Kizil, C., Kyritsis, N., Dudczig, S., Kroehne, V., Freudenreich, D., Kaslin, J. and Brand, M.
  
• Age-related islet autoantibody incidence in offspring of patients with type 1 diabetes. Diabetologia. 2012 Jul;55(7):1937-43
  Ziegler AG, Bonifacio E; BABYDIAB-BABYDIET Study Group
  
• Reduced-intensity conditioning versus standard conditioning before allogeneic haemopoietic cell transplantation in patients with acute myeloid leukaemia in first complete remission: a prospective, open-label randomised phase 3 trial. Lancet Oncol. 2012 Oct;13(10):1035-44
  Bornhäuser M, Kienast J, Trenschel R, Burchert A, Hegenbart U, Stadler M, Baurmann H, Schäfer-Eckart K, Holler E, Kröger N, Schmid C, Einsele H, Kiehl MG, Hiddemann W, Schwerdtfeger R, Buchholz S, Dreger P, Neubauer A, Berdel WE, Ehninger G, Beelen DW, Schetelig J, Stelljes M
  
• (2013) Regulation of cerebral cortex size and folding by expansion of basal progenitors. EMBO J, 32:1817-28
  Nonaka-Kinoshita M, Reillo I, Artegiani B, Martinez M, Nelson M, Borrell V and Calegari F
  
• (2013) Sclerostin antibody treatment improves bone mass, bone strength, and bone defect regeneration in rats with type 2 diabetes mellitus. J Bone Miner Res. 28:627-38
  Hamann C, Rauner M, Höhna Y, Bernhardt R, Mettelsiefen J, Goettsch C, Günther KP, Stolina M, Han CY, Asuncion FJ, Ominsky MS, Hofbauer LC
  
• (2013) Transcriptome sequencing during mouse brain development identifies long non-coding RNAs functionally involved in neurogenic commitment. EMBO J, 32:3145-60
  Aprea J, Prenninger S, Dori M, Sebastian Monasor L, Wessendorf E, Zocher S, Massalini S, Ghosh T, Alexopoulou D, Lesche M, Dahl A, Groszer M, Hiller M and Calegari F
  
• (2013). Assessing computational methods for transcription factor target gene identification based on ChIP-seq data. PLoS Comput Biol 9, e1003342
  Sikora-Wohlfeld, W., Ackermann, M., Christodoulou, E.G., Singaravelu, K., and Beyer, A.
  
• (2013). CD133 is a modifier of hematopoietic progenitor frequencies but is dispensable for the maintenance of mouse hematopoietic stem cells. Proc Natl Acad Sci USA. 110(14):5582-7
  Arndt K, Grinenko T, Mende N, Reichert D, Portz M, Ripich T, Carmeliet P, Corbeil D, Waskow C
  
• (2013). Characterization of light lesion paradigms and optical coherence tomography as tools to study adult retina regeneration in zebrafish. PLoS One 8, e80483
  Weber, A., Hochmann, S., Cimalla, P., Gartner, M., Kuscha, V., Hans, S., Geffarth, M., Kaslin, J., Koch, E., and Brand, M.
  
• (2013). Defined polymer-peptide conjugates to form cell-instructive starPEG-heparin matrices in situ. Adv Mater 25, 2606-2610
  Tsurkan, M.V., Chwalek, K., Prokoph, S., Zieris, A., Levental, K.R., Freudenberg, U., and Werner, C.
  
• (2013). Development and specification of cerebellar stem and progenitor cells in zebrafish: from embryo to adult. Neural Dev 8, 9
  Kaslin, J., Kroehne, V., Benato, F., Argenton, F., and Brand, M.
  
• (2013). Emergence of Individuality in Genetically Identical Mice. Science 340, 6133: 756-9
  Freund, J., A. M. Brandmaier, L. Lewejohann, I. Kirste, M. Kritzler, A. Kruger, N. Sachser, U. Lindenberger, and G. Kempermann
  
• (2013). Progressive specification rather than intercalation of segments during limb regeneration. Science 342, 1375-1379
  Roensch, K., Tazaki, A., Chara, O., and Tanaka, E.M.
  
• (2013). Seroconversion to multiple islet autoantibodies and risk of progression to diabetes in children. JAMA 309, 2473-2479
  Ziegler, A.G., Rewers, M., Simell, O., Simell, T., Lempainen, J., Steck, A., Winkler, C., Ilonen, J., Veijola, R., Knip, M., Bonifacio, E., and Eisenbarth, G.S.
  
• (2013). Transplantation of human islets without immunosuppression. Proc Natl Acad Sci USA 110, 19054-19058
  Ludwig, B., Reichel, A., Steffen, A., Zimerman, B., Schally, A. V., Block, N. L., Colton, C. K., Ludwig, S., Kersting, S., Bonifacio, E., Solimena, M., Gendler, Z., Rotem, A., Barkai, U., and Bornstein, S. R.
  
• (2013). Transposon mediated BAC transgenesis via pronuclear injection of mouse zygotes. Genesis 51, 135-141
  Rostovskaya, M., Naumann, R., Fu, J., Obst, M., Mueller, D., Stewart, A.F., and Anastassiadis, K.
  
• (2014) Clonal expansion capacity defines two consecutive developmental stages of long-term hematopoietic stem cells. J Exp Med. 2014 Feb 10;211(2):209-15
  Grinenko T, Arndt K, Portz M, Mende N, Günther M, Cosgun KN, Alexopoulou D, Lakshmanaperumal N, Henry I, Dahl A, Waskow C
  
• (2014) CXCR4 blockade and sphingosine-1-phosphate activation facilitate engraftment of haematopoietic stem and progenitor cells in a non-myeloablative transplant model. Br J Haematol. 164(3):409-13
  Shayegi N, Meyer C, Lambert K, Ehninger G, Brand M, Bornhäuser M
  
• (2014) Kit regulates HSC engraftment across the humanmouse species barrier. Cell Stem Cell 7;15(2):227-38
  Cosgun KN, Rahmig S, Mende N, Reinke S, Hauber I, Schäfer C, Petzold A, Weisbach H, Heidkamp G, Purbojo A, Cesnjevar R, Platz A, Bornhäuser M, Schmitz M, Dudziak D, Hauber J, Kirberg J, Waskow C
  
• (2014) Letermovir for cytomegalovirus prophylaxis in hematopoietic-cell transplantation. N Engl J Med. 8;370(19):1781-9
  Chemaly RF, Ullmann AJ, Stoelben S, Richard MP, Bornhäuser M, Groth C, Einsele H, Silverman M, Mullane KM, Brown J, Nowak H, Kölling K, Stobernack HP, Lischka P, Zimmermann H, Rübsamen-Schaeff H, Champlin RE, Ehninger G; AIC246 Study Team
  
• (2014). Fundamental differences in dedifferentiation and stem cell recruitment during skeletal muscle regeneration in two salamander species. Cell Stem Cell 14, 174-187
  Sandoval-Guzman, T., Wang, H., Khattak, S., Schuez, M., Roensch, K., Nacu, E., Tazaki, A., Joven, A., Tanaka, E.M., and Simon, A.
  
• (2014). Glycosaminoglycanbased hydrogels to modulate heterocellular communication in in vitro angiogenesis models. Scientific Reports 4
  Chwalek, K., Tsurkan, M.V., Freudenberg, U., and Werner, C.
  
• (2014). Neuroinflammation and central nervous system regeneration in vertebrates. Trends in Cell Biology
  Kyritsis, N., Kizil, C. and Brand, M.
  
• (2014). Using pancreas tissue slices for in situ studies of islet of Langerhans and acinar cell biology. Nature protocols, 9(12), 2809–2822
  Marciniak, A., Cohrs, C. M., Tsata, V., Chouinard, J. A., Selck, C., Stertmann, J., Reichelt, S., Rose, T., Ehehalt, F., Weitz, J., Solimena, M., Slak Rupnik, M. and Speier, S.
  
• 2014. Calcineurin regulates coordinated outgrowth of zebrafish regenerating fins. Dev Cell 28, 573-587
  Kujawski, S., Lin, W., Kitte, F., Bormel, M., Fuchs, S., Arulmozhivarman, G., Vogt, S., Theil, D., Zhang, Y., Antos, C.L.
  
• (2015) CCND1-CDK4-mediated cell cycle progression provides a competitive advantage for human hematopoietic stem cells in vivo. J Exp Med. ;212(8):1171-83
  Mende N, Kuchen EE, Lesche M, Grinenko T, Kokkaliaris KD, Hanenberg H, Lindemann D, Dahl A, Platz A, Höfer T, Calegari F, Waskow C
  
• (2015). Adoptive transfer of allogeneic regulatory T cells into patients with chronic graft-versus-host disease. Cytotherapy 17, 473-486
  Theil, A., Tuve, S., Oelschlagel, U., Maiwald, A., Dohler, D., Ossmann, D., Zenkel, A., Wilhelm, C., Middeke, J.M., Shayegi, N., et al.
  
• (2015). Distinct roles of beta-cell mass and function during type 1 diabetes onset and remission. Diabetes 64, 2148-2160
  Chmelova, H., Cohrs, C.M., Chouinard, J.A., Petzold, C., Kuhn, M., Chen, C., Roeder, I., Kretschmer, K., and Speier, S.
  
• (2015). Effects of high-dose oral insulin on immune responses in children at high risk for type 1 diabetes: the Pre-POINT randomized clinical trial. JAMA 313, 1541
  Bonifacio E, Ziegler AG, Klingensmith G, Schober E, Bingley PJ, Rottenkolber M, Theil A, Eugster A, Puff R, Peplow C, Buettner F, Lange K, Hasford J, Achenbach P, and the Pre- POINT Study Group
  
• (2015). Human-specific gene ARHGAP11B promotes basal progenitor amplification and neocortex expansion. Science 347, 1465-1470
  Florio, M., Albert, M., Taverna, E., Namba, T., Brandl, H., Lewitus, E., Haffner, C., Sykes, A., Wong, F.K., Peters, J., et al.
  
• (2015). No Evidence for Cardiomyocyte Number Expansion in Preadolescent Mice. Cell 163, 1026-1036
  Alkass, K., Panula, J., Westman, M., Wu, T.D., Guerquin-Kern, J.L., and Bergmann, O.
  
• (2015). Planar cell polarity-mediated induction of neural stem cell expansion during axolotl spinal cord regeneration. Elife 4, e10230
  Rodrigo Albors, A., Tazaki, A., Rost, F., Nowoshilow, S., Chara, O., and Tanaka, E.M.
  
• (2015). RNAi profiling of primary human AML cells identifies ROCK1 as a therapeutic target and nominates fasudil as an antileukemic drug. Blood 125, 3760-3768
  Wermke, M., Camgoz, A., Paszkowski-Rogacz, M., Thieme, S., von Bonin, M., Dahl, A., Platzbecker, U., Theis, M., Ehninger, G., Brenner, S., et al.
  
• (2015). Tox: a multifunctional transcription factor and novel regulator of mammalian corticogenesis. EMBO J 34, 896-910
  Artegiani, B., de Jesus Domingues, A.M., Bragado Alonso, S., Brandl, E., Massalini, S., Dahl, A., and Calegari, F.
  
• Structural and functional insights into sclerostin-glycosaminoglycan interactions in bone. Biomaterials. 2015 Jul 15;67:335-345
  Salbach-Hirsch J, Samsonov SA, Hintze V, Picke AK, Rauner M, Gehrcke JP, Moeller S, Schnabelrauch M, Scharnweber D, Pisabarro MT, Hofbauer LC
  
• (2016). Aldehyde dehydrogenase activity is necessary for beta cell development and functionality in mice. Diabetologia 59, 139-150
  Anastasiou, V., Ninou, E., Alexopoulou, D., Stertmann, J., Muller, A., Dahl, A., Solimena, M., Speier, S., Serafimidis, I., and Gavalas, A.
  
• (2016). FGF8 and SHH substitute for anterior-posterior tissue interactions to induce limb regeneration. Nature 533, 407-410
  Nacu, E., Gromberg, E., Oliveira, C.R., Drechsel, D., and Tanaka, E.M.
  
• (2016). Identification of proliferative and mature β-cells in the islets of Langerhans. Nature 535(7612):430-4
  Bader E, Migliorini A, Gegg M, … , Chmelova H, Chouinard JA, Oskolkov N, Korsgren O, Speier S, Lickert H
  
• (2016). Live Imaging of Axolotl Digit Regeneration Reveals Spatiotemporal Choreography of Diverse Connective Tissue Progenitor Pools. Dev Cell 39, 411-423
  Currie, J.D., Kawaguchi, A., Traspas, R.M., Schuez, M., Chara, O., and Tanaka, E.M.
  
• (2016). MARCKS-like protein is an initiating molecule in axolotl appendage regeneration. Nature 531, 237-240
  Sugiura, T., Wang, H., Barsacchi, R., Simon, A., and Tanaka, E.M.
  
• (2016). Mesenchymal Stromal Cells for Treatment of Acute Steroid-Refractory Graft Versus Host Disease: Clinical Responses and Long-Term Outcome. Stem Cells 34, 357-366
  von Dalowski, F., Kramer, M., Wermke, M., Wehner, R., Rollig, C., Alakel, N., Stolzel, F., Parmentier, S., Sockel, K., Krech, M., et al.
  
• (2016). Retinal Organoids from Pluripotent Stem Cells Efficiently Recapitulate Retinogenesis. Stem Cell Reports 6, 4: 525-38
  Volkner, M., M. Zschatzsch, M. Rostovskaya, R. W. Overall, V. Busskamp, K. Anastassiadis, and M. O. Karl
  
• Directed evolution of a recombinase that excises the provirus of most HIV-1 primary isolates with high specificity. Nat Biotechnol. 2016 Apr;34(4):401-9
  Karpinski J, Hauber I, Chemnitz J, Schäfer C, Paszkowski-Rogacz M, Chakraborty D, Beschorner N, Hofmann-Sieber H, Lange UC, Grundhoff A, Hackmann K, Schrock E, Abi- Ghanem J, Pisabarro MT, Surendranath V, Schambach A, Lindner C, van Lunzen J, Hauber J, Buchholz F
  
• Increased EPO Levels Are Associated with Bone Loss in Mice Lacking PHD2 in EPO-Producing Cells. J Bone Miner Res. 31(10):1877-1887, 2016
  Rauner M, Franke K, Murray M, Singh RP, Hiram-Bab S, Platzbecker U, Gassmann M, Socolovsky M, Neumann D, Gabet Y, Chavakis T, Hofbauer LC, Wielockx B
  
• Retinal Transplantation of Photoreceptors Results in Donor-Host Cytoplasmic Exchange. (2016). Nat Commun 7: 13028
  Santos-Ferreira, T., S. Llonch, O. Borsch, K. Postel, J. Haas, and M. Ader
  
• (2017) Immune suppressive and bone inhibitory effects of prednisolone in growing and regenerating zebrafish tissues. J Bone Miner Res. 32:2476-88
  Geurtzen K, Vernet A, Freidin A, Rauner M, Hofbauer LC, Schneider JE, Brand M, Knopf F
  
• (2017). A divergent population of autoantigen-responsive CD4+ T cells in infants prior to beta cell autoimmunity. Sci Transl Med 9
  Heninger AK, Eugster A, Kuehn D, Buettner F, Kuhn M, Lindner A, Dietz S, Jergens S, Wilhelm C, Beyerlein A, Ziegler AG, Bonifacio E
  
• (2017). A Global Approach for Quantitative Super Resolution and Electron Microscopy on Cryo and Epoxy Sections Using Self-labeling Protein Tags. Sci Rep 7, 23
  Muller, A., Neukam, M., Ivanova, A., Sonmez, A., Munster, C., Kretschmar, S., Kalaidzidis, Y., Kurth, T., Verbavatz, J.M., and Solimena, M.
  
• (2017). Clonal fate-mapping quantifies the number of haematopoietic stem cells that arise during development. Nature Cell Biology, 19(1):17-27
  Henninger, J., Santoso, B., Hans, S., Durand, E., Moore, J., Mosimann, C., Brand, M., Traver, D., and Zon, L.
  
• (2017). Combined influence of biophysical and biochemical cues on maintenance and proliferation of hematopoietic stem cells. Biomaterials 138, 108-117
  Gvaramia, D., Muller, E., Muller, K., Atallah, P., Tsurkan, M., Freudenberg, U., Bornhauser, M., and Werner, C.
  
• (2017). Different developmental histories of beta-cells generate functional and proliferative heterogeneity during islet growth. Nat Commun 8, 664
  Singh, S.P., Janjuha, S., Hartmann, T., Kayisoglu, O., Konantz, J., Birke, S., Murawala, P., Alfar, E.A., Murata, K., Eugster, A., Tsuji N, Morrissey ER, Brand M, Ninov N.
  
• (2017). Distinct roles of neuroepithelial-like and radial glia-like progenitor cells in cerebellar regeneration. Development, 144, 1462-1471
  Kaslin, J., Kroehne, V., Ganz, J., Hans, S. and Brand, M.
  
• (2017). Favorable outcome of experimental islet xenotransplantation without immunosuppression in a nonhuman primate model of diabetes. PNAS USA, 114(44), 11745–11750
  Ludwig, B., Ludwig, S., Steffen, A., Knauf, Y., Zimerman, B., …, Colton, C. K., Bonifacio, E., Solimena, M., Reichel, A., Schally, A. V., Rotem, A., Barkai, U., Grinberg-Rashi, H., Kaup, F. J., …, Avni, Y., Jones, P., Bornstein, S. R.
  
• (2017). Heparinbased hydrogels induce human renal tubulogenesis in vitro. Acta Biomater
  Weber, H.M., Tsurkan, M.V., Magno, V., Freudenberg, U., and Werner, C.
  
• (2017). On-demand optogenetic activation of human stem-cell-derived neurons. Sci Rep 7, 14450
  Klapper, S.D., Sauter, E.J., Swiersy, A., Hyman, M.A.E., Bamann, C., Bamberg, E., and Busskamp, V.
  
• (2017). Pancreas lineage allocation and specification are regulated by sphingosine-1-phosphate signalling. PLoS Biol 15, e2000949
  Serafimidis, I., Rodriguez-Aznar, E., Lesche, M., Yoshioka, K., Takuwa, Y., Dahl, A., Pan, D., and Gavalas, A.
  
• (2017). Serum Proteases Potentiate BMP- Induced Cell Cycle Re-entry of Dedifferentiating Muscle Cells during Newt Limb Regeneration. Dev Cell 40, 608-617 e606
  Wagner, I., Wang, H., Weissert, P.M., Straube, W.L., Shevchenko, A., Gentzel, M., Brito, G., Tazaki, A., Oliveira, C., Sugiura, T., et al.
  
• (2017). T cell receptor repertoires after adoptive transfer of expanded allogeneic regulatory T cells. Clin Exp Immunol 187, 316-324
  Theil, A., Wilhelm, C., Kuhn, M., Petzold, A., Tuve, S., Oelschlagel, U., Dahl, A., Bornhauser, M., Bonifacio, E., and Eugster, A.
  
• (2017). Zebrafish In-vivo Screening for Compounds Enhancing Hematopoiesis: Preclinical Validation in Human CD34+ Stem and Progenitor Cells. Scientific Rep., 21;7(1):12084
  Arulmozhivarman, G., Kraeter, M., Wobus, M., Friedrichs, J., Bejestani, E., Müller, K., Lambert, K., Alexopoulou, D., Dahl, A., Stöter, M., Bickle, M., Shayegi, N., Hampe, J., Stölzel, F., Brand, M., von Bonin, M. and Bornhäuser, M.
  
• 2017. Conserved and novel functions of programmed cellular senescence during vertebrate development. Development 144, 106- 114
  Davaapil, H., Brockes, J.P., Yun, M.H.
  
• Del-1 is a novel component of the hematopoietic stem cell niche and regulates steady-state and emergency myelopoiesis. J. Clin. Invest. 127:3624-3639, 2017
  Mitroulis I, Chen L-S, Singh RP, Kourtzelis I, Economopoulou M, Kajikawa T, Troullinaki M, Ziogas A, Ruppova K, Hosur K, Maekawa T, Wang B, Subramanian P, Tonn T, Di Scala M, Hidalgo A, Wielockx B, Hajishengallis G, Chavakis T
  
• Prediction of fractures and major cardiovascular events in men using serum osteoprotegerin levels: The prospective STRAMBO study. J Bone Miner Res. 2017;32:2288-96
  Szulc P, Chapurlat R, Hofbauer LC
  
• (2018) Application and optimization of CRISPR-Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum). Nat Protoc. 13(12):2908-2943
  Fei JF, Lou WP, Knapp D, Murawala P, Gerber T, Taniguchi Y, Nowoshilow S, Khattak S, Tanaka EM
  
• (2018) Critical Role of TGF-β and IL-2 Receptor Signaling in Foxp3 Induction by an Inhibitor of DNA Methylation. Front Immunol. 9:125
  Freudenberg K, Lindner N, Dohnke S, Garbe AI, Schallenberg S, Kretschmer K
  
• (2018). 3D Culture Method for Alzheimer's Disease Modeling Reveals Interleukin-4 Rescues Abeta42-Induced Loss of Human Neural Stem Cell Plasticity. Dev Cell 46, 85-101 e108
  Papadimitriou, C., Celikkaya, H., Cosacak, M.I., Mashkaryan, V., Bray, L., Bhattarai, P., Brandt, K., Hollak, H., Chen, X., He, S., et al.
  
• (2018). Age-related islet inflammation marks the proliferative decline of pancreatic beta-cells in zebrafish. Elife 7
  Janjuha, S., Singh, S.P., Tsakmaki, A., Mousavy Gharavy, S.N., Murawala, P., Konantz, J., Birke, S., Hodson, D.J., Rutter, G.A., Bewick, G.A., et al.
  
• (2018). Defined Geldrop Cultures Maintain Neural Precursor Cells. Sci Rep 8, 8433
  Vogler, S., Prokoph, S., Freudenberg, U., Binner, M., Tsurkan, M., Werner, C., and Kempermann, G.
  
• (2018). Impaired DNA damage response signaling by FUS-NLS mutations leads to neurodegeneration and FUS aggregate formation. Nature communications, 9(1), 335
  Naumann, M., Pal, A., Goswami, A., Lojewski, X., Japtok, J., Vehlow, A., Naujock, M., Günther, R., Jin, M., Stanslowsky, N., Reinhardt, P., Sterneckert, J., Frickenhaus, M., Pan- Montojo, F., Storkebaum, E., Poser, I., Freischmidt, A., Weishaupt, J. H., Holzmann, K., Troost, D., … Hermann, A.
  
• (2018). Machine learning based classification of cells into chronological stages using single-cell transcriptomics. Sci Rep 8, 17156
  Singh, S.P., Janjuha, S., Chaudhuri, S., Reinhardt, S., Krankel, A., Dietz, S., Eugster, A., Bilgin, H., Korkmaz, S., Zararsiz, G., et al.
  
• (2018). Modulation of Myelopoiesis Progenitors Is an Integral Component of Trained Immunity. Cell, 172(1-2), 147–161.e12
  Mitroulis, I., Ruppova, K., Wang, B., Chen, L. S., Grzybek, M., Grinenko, T., Eugster, A., Troullinaki, M., Palladini, A., Kourtzelis, I., Chatzigeorgiou, A., Schlitzer, A., Beyer, M., Joosten, L., Isermann, B., Lesche, M., Petzold, A., Simons, K., Henry, I., Dahl, A., … Chavakis, T.
  
• (2018). Simultaneous lineage tracing and cell-type identification using CRISPR-Cas9- induced genetic scars. Nat Biotechnol 36, 469-473
  Spanjaard, B., Hu, B., Mitic, N., Olivares-Chauvet, P., Janjuha, S., Ninov, N., and Junker, J.P.
  
• (2018). Single-cell analysis uncovers convergence of cell identities during axolotl limb regeneration. Science 362
  Gerber, T., Murawala, P., Knapp, D., Masselink, W., Schuez, M., Hermann, S., Gac-Santel, M., Nowoshilow, S., Kageyama, J., Khattak, S., Currie, J.D., Camp, J.G., Tanaka, E.M., Treutlein, B.
  
• (2018). Systems biology of the IMIDIA biobank from organ donors and pancreatectomised patients defines a novel transcriptomic signature of islets from individuals with type 2 diabetes. Diabetologia 61, 641-657
  Solimena, M., Schulte, A.M., Marselli, L., Ehehalt, F., Richter, D., Kleeberg, M., Mziaut, H., Knoch, K.P., Parnis, J., Bugliani, M., et al.
  
• (2018). The axolotl genome and the evolution of key tissue formation regulators. Nature 554, 50-55
  Nowoshilow, S., Schloissnig, S., Fei, J.F., Dahl, A., Pang, A.W.C., Pippel, M., Winkler, S., Hastie, A.R., Young, G., Roscito, J.G., Falcon, F., Knapp, D., Powell, S., Cruz, A., Cao, H., Habermann, B., Hiller, M., Tanaka, E.M., Myers, E.W.
  
• Genetic scores to stratify risk of developing multiple islet autoantibodies and type 1 diabetes: A prospective study in children. PLoS Med. 2018 Apr 3;15(4):e1002548
  Bonifacio E, Beyerlein A, Hippich M, Winkler C, Vehik K, Weedon MN, Laimighofer M, Hattersley AT, Krumsiek J, Frohnert BI, Steck AK, Hagopian WA, Krischer JP, Lernmark Å, Rewers MJ, She JX, Toppari J, Akolkar B, Oram RA, Rich SS, Ziegler AG; TEDDY Study Group
  
• Long-term efficacy of reduced-intensity versus myeloablative conditioning before allogeneic haemopoietic cell transplantation in patients with acute myeloid leukaemia in first complete remission: retrospective follow-up of an open-label, randomised phase 3 trial. Lancet Haematol. 2018 Apr;5(4):e161-e169
  Fasslrinner F, Schetelig J, Burchert A, Kramer M, Trenschel R, Hegenbart U, Stadler M, Schäfer-Eckart K, Bätzel M, Eich H, Stuschke M, Engenhart-Cabillic R, Krause M, Dreger P, Neubauer A, Ehninger G, Beelen D, Berdel WE, Siepmann T, Stelljes M, Bornhäuser M
  
• (2019) Aldh1b1 expression defines progenitor cells in the adult pancreas and is required for Kras-induced pancreatic cancer. Proc Natl Acad Sci U S A. 2019 Sep 23
  Mameishvili E, Serafimidis I, Iwaszkiewicz S, Lesche M, Reinhardt S, Bölicke N, Büttner M, Stellas D, Papadimitropoulou A, Szabolcs M, Anastassiadis K, Dahl A, Theis F, Efstratiadis A, Gavalas A
  
• (2019) An increase in neural stem cells and olfactory bulb adult neurogenesis improves discrimination of highly similar odorants. EMBO J, embj.201798791
  Bragado Alonso S, Reinert JK, Marichal N, Massalini S, Berninger B, Kuner T, Calegari F
  
• (2019). 3D Bioprinting of Functional Islets of Langerhans in an Alginate/Methylcellulose Hydrogel Blend. Adv Healthc Mater 8, e1801631
  Duin, S., Schutz, K., Ahlfeld, T., Lehmann, S., Lode, A., Ludwig, B., and Gelinsky, M.
  
• (2019). Automated Clinical Grade Expansion of Regulatory T Cells in a Fully Closed System. Frontiers in Immunology, 10, 38
  Marín Morales, J. M., Münch, N., Peter, K., Freund, D., Oelschlägel, U., Hölig, K., Böhm, T., Flach, A. C., Keßler, J., Bonifacio, E., Bornhäuser, M., and Fuchs, A.
  
• (2019). Dual Inhibition of GSK3beta and CDK5 Protects the Cytoskeleton of Neurons from Neuroinflammatory- Mediated Degeneration In Vitro and In Vivo. Stem Cell Reports 12, 502-517
  Reinhardt, L., Kordes, S., Reinhardt, P., Glatza, M., Baumann, M., Drexler, H.C.A., Menninger, S., Zischinsky, G., Eickhoff, J., Frob, C., et al.
  
• (2019). Foxp3+ Regulatory T Cells in Bone and Hematopoietic Homeostasis. Front Endocrinol. 10:578
  Fischer L, Herkner C, Kitte R, Dohnke S, Riewaldt J, Kretschmer K, Garbe AI
  
• (2019). Glucocorticoid treatment leads to aberrant ion and macromolecular transport mechanisms in regenerating zebrafish fins. Front Endocrinol
  Schmidt JR, Geurtzen K, von Bergen M, Schubert K and Knopf F
  
• (2019). Modelling pancreatic β-cell inflammation in zebrafish identifies the natural product wedelolactone for human islet protection. Dis Model Mech. 12
  Delgadillo-Silva LF, Tsakmaki A, Akhtar N, Franklin ZJ, Konantz J, Bewick GA, Ninov N
  
• (2019). Polyacrylamide Bead Sensors for in vivo Quantification of Cell-Scale Stress in Zebrafish Development. Nature Commun 10, 1-11
  Träber, N., Uhlmann, K., Girardo, S., Kesavan, G., Wagner, K., Friedrichs, J., Goswami, R., Bai, K., Brand, M., Werner, C., et al.
  
• (2019). Protein Methyltransferase Inhibition Decreases Endocrine Specification Through the Upregulation of Aldh1b1 Expression. Stem Cells 37, 640-651
  Giannios, I., Serafimidis, I., Anastasiou, V., Pezzolla, D., Lesche, M., Andree, C., Bickle, M., and Gavalas, A.
  
• Anti-CD20 immunotherapy as a bridge to tolerance, after allogeneic stem cell transplantation for patients with chronic lymphocytic leukaemia: results of the CLLX4 trial. Br J Haematol. 2019 Mar;184(5):833-836
  Schetelig J, Link CS, Stuhler G, Wagner EM, Hänel M, Kobbe G, Böttcher S, Kreuzer KA, Middeke JM, Sockel K, Teipel R, von Bonin M, Stölzel F, Kramer M, Stilgenbauer S, Hallek M, Bornhäuser M; Deutsche CLL Studiengruppe and the German Cooperative Transplant Study Group
  
• Leader β-cells coordinate Ca2+ dynamics across pancreatic islets in vivo. Nature Metab. 2019 (6):615-629
  Salem V, Silva LD, Suba K, Georgiadou E, Neda Mousavy Gharavy S, Akhtar N, Martin- Alonso A, Gaboriau DCA, Rothery SM, Stylianides T, Carrat G, Pullen TJ, Singh SP, Hodson DJ, Leclerc I, Shapiro AMJ, Marchetti P, Briant LJB, Distaso W, Ninov N, Rutter GA
  
• Transferrin receptor 2 controls bone mass and pathological bone formation via BMP and Wnt signaling. Nature Metabolism 2019; 1:111–24
  Rauner M, Baschant U, Roetto A, Pellegrino RM, Rother S, Salbach-Hirsch J, Weidner H, Hintze V, Campbell G, Petzold A, Lemaitre R, Henry I, Bellido T, Theurl I, Altamura S, Colucci S, Muckenthaler MU, Schett G, Komla-Ebri DSK, Bassett JHD, Williams GR, Platzbecker U & Hofbauer LC
  
• (2020). Long-term in vivo imaging reveals tumor-specific dissemination and captures host tumor interaction in zebrafish xenografts. Scientific Rep.. 6;10(1):13254
  Asokan N, Daetwyler S, Bernas SN, Schmied C, Vogler S, Lambert K, Wobus M, Wermke M, Kempermann G, Huisken J, Brand M, Bornhäuser M
  
• (2020). Single cell sequencing of radial glia progeny reveals diversity of newborn neurons in the adult zebrafish brain. Development 147
  Lange, C.; Fabian Rost, Anja Machate, Susanne Reinhardt, Matthias Lesche, Anke Weber, Veronika Kuscha, Andreas Dahl, Steffen Rulands and Brand, M.
  
• Epithelial calcineurin controls microbiota-dependent intestinal tumor development. Nat Med. 2016 May;22(5):506-15
  Peuker K, Muff S, Wang J, Künzel S, Bosse E, Zeissig Y, Luzzi G, Basic M, Strigli A, Ulbricht A, Kaser A, Arlt A, Chavakis T, van den Brink GR, Schafmayer C, Egberts JH, Becker T, Bianchi ME, Bleich A, Röcken C, Hampe J, Schreiber S, Baines JF, Blumberg RS, Zeissig S