Aufklärung von Mechanismen der kälteinduzierten chemo-elektrischen systemischen Signalleitung und der Integration des Two Pore Channel 1 im Signalnetzwerk

• Antragsteller: Professor Dr. Edgar Peiter
• Fachliche Zuordnung: Biochemie und Biophysik der Pflanzen ; Pflanzenphysiologie
• Förderung: Förderung von 2017 bis 2023
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 327830483

Plants that face a localized stress or attack may launch responses in unaffected plant parts, which braces the whole plant for the anticipated problem. A signalling system to rapidly convey this information within the plant involves calcium (Ca2+), reactive oxygen species (ROS), and electrical potential waves. Albeit it is becoming apparent that this rapid "chemo-electric systemic signalling" (CHESS) is pivotal to integrate whole-plant responses, its mechanistic basis is poorly understood. The overarching objective of this proposal package is therefore to decipher general and stimulus-specific mechanisms of rapid systemic signalling. Within this framework, this project focuses on (1) the characterization of cold-triggered systemic Ca2+ signals and the identification of genes involved in their generation, (2) the determination of systemic Ca2+ signatures that may encode stimulus-specific information, and (3) the elucidation how the vacuolar Two Pore Channel 1, a key player in systemic Ca2+ signalling, is integrated in this signalling network. A combination of forward and reverse genetic approaches will be employed to identify and characterize key players in the generation, transmission, and decoding of rapid chemo-electric signals. The close interaction of groups focussing on electric, ROS, and Ca2+ signalling, as well as those working on downstream responses, will allow to build a conceptual framework of rapid signalling processes in plants. Furthermore, the translation of this knowledge into the improvement of cereal crops will be initiated in this project.

• DFG-Verfahren: Sachbeihilfen
• Internationaler Bezug: Indien
• Partnerorganisation: Department of Biotechnology (DBT) ; Ministry of Science and Technology
• Kooperationspartnerinnen / Kooperationspartner: Professor Dr. Shree Prakash Pandey; Dr. Jyothilakshmi Vadassery; Dr. Radhika Venkatesan