The circadian feedback module - assembly and localization as crucial events for circadian dynamics (D02)

• Subject Area: Biochemistry
• Term: from 2007 to 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 25065445

Mammalian cells generate molecular ~24-hour rhythms using a multi-protein complex that eventually negatively regulates the expression of its components. Since the dynamics of this mechanism is poorly understood, we propose to study: (i) the role of transportin1-mediated nuclear transport using FRAP and interaction experiments; (ii) the role of COPI vesicles for module assembly using FRET, co-immunoprecipitation and mass spectrometry; and (iii) the identity and dynamics of module composition using quantitative mass spectrometry and subsequent functional analyses.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 740: From Molecules to Modules: Organisation and Dynamics of Functional Units in Cells
• Applicant Institution: shared FU Berlin and HU Berlin through:; Charité - Universitätsmedizin Berlin
• Project Head: Professor Dr. Achim Kramer