Regulatory phosphorylation of scaffolding proteins of the inside-out-signaling complex (D04+)

• Subject Area: Biochemistry
• Term: from 2007 to 2010
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 25065445

The project D4 (Höfer/Freund) integrates experimentation and modeling to elucidate how phosphorylations of scaffolding proteins cause the reversible assembly of a module that regulates T cell adhesion. The interactions of the scaffolds ADAP and Vav with the Src-family kinase Fyn and the phosphatase SHP-2 as well as the resulting phosphorylation dynamics are studied in a reconstituted system and in the cellular context. The modeling makes testable predictions on the role of competing feedback loops for kinase and phosphatase activation and the control exerted on signal transduction by individual protein binding and phosphorylation steps.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 740: From Molecules to Modules: Organisation and Dynamics of Functional Units in Cells
• Applicant Institution: shared FU Berlin and HU Berlin through:; Charité - Universitätsmedizin Berlin
• Co-Applicant Institution: Freie Universität Berlin; Humboldt-Universität zu Berlin
• Project Heads: Professor Dr. Christian Freund; Professor Dr. Thomas Höfer, until 2/2007