Molecular electron microscopy (Z01)

• Subject Area: Structural Biology
• Term: from 2007 to 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 25065445

Project Z01 aims to support the structural analysis of functional modules within the consortium using cryo-electron microscopy (cryo-EM). We first apply routine screening techniques to assess the sample quality followed by automated data collection of negatively stained samples or samples embedded in vitrified ice. In the next step we support the determination of intitial structure models followed by an in-depth cryo-EM analysis, which will be done in close collaboration with the individual partners. Furthermore, our project also aims to develop and implement new methodologies focusing on the in silico analysis of sample heterogeneity. Additionally, we now also offer automated data acquisition for cell biological applications and cellular electron tomography.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 740: From Molecules to Modules: Organisation and Dynamics of Functional Units in Cells
• Applicant Institution: shared FU Berlin and HU Berlin through:; Charité - Universitätsmedizin Berlin
• Co-Applicant Institution: Max-Planck-Institut für molekulare Genetik (MPIMG)
• Project Heads: Dr. Thorsten Mielke; Professor Dr. Christian M. T. Spahn