Chromatin as limiting factor in the regulation of DNA repair pathway choice (A23*)

• Subject Area: Biochemistry
• Term: from 2017 to 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 213249687

Cells use different pathways for the repair of DNA double strand breaks. Pathway choice is strictly regulated during the cell cycle, since DNA ends are differentially processed at different cell cycle stages by a process called DNA end resection. Our preliminary data suggest that also the activity of nucleosome remodellers on damaged chromatin is a limiting factor in the regulation of DNA end resection during the cell cycle. We will therefore use the S. cerevisiae model to reveal the nature of the resection-permissive or –restrictive state and critical activities of nucleosome remodellers. Moreover, we will use in vitro reconstitution to investigate the mechanism of resection inhibition.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1064: Chromatin Dynamics
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Head: Professor Dr. Boris Pfander