InflammatorySystemsMicrobiome: Systems diagnostic of the human gut microbiome on inflammatory diseases using metaproteome analysis

• Applicant: Professor Dr.-Ing. Robert Heyer
• Subject Area: Parasitology and Biology of Tropical Infectious Disease Pathogens ; Biological Process Engineering
• Term: from 2017 to 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 391056443

The aim of this project is to investigate the interactions of gut inflammation and the microbiome based on metaproteome analysis of 100 human fecal samples. Thereby the impact of the microbiome on the pathogenesis of inflammatory bowel diseases and (pre)malignant transformation will be elucidated. Furthermore, patterns of marker proteins will be identified by comprehensive biostatistical and medical data evaluation.The long term goal of this project is to establish proteotyping of human fecal samples in analogy to MALDI-MS based identification of microorganisms (Welker and Moore, 2011, Karlsson, et al., 2015) as a routine tool for diagnostics of inflammatory processes related to the gut microbiome in laboratory medicine. In order to achieve this long term goal this project should prove the presence of disease specific pattern within the metaproteome analyses of fecal samples. The targeted clinical benefit of this approach is to gain non-invasive knowledge about the inflammatory status of the human gut system.

• DFG Programme: Research Grants
• Co-Investigators: Professor Dr. Lars Peter Bechmann (†); Professor Dr. Dirk Benndorf; Professor Dr. Ali Canbay; Professor Dr.-Ing. Udo Reichl; Professor Dr. Christian Schulz; Dr. Ramiro Vilchez-Vargas