Characterization of the DOG1 and ABA molecular interplay in seed dormancy.
Plant Biochemistry and Biophysics
Final Report Abstract
The interconnected challenges of climate change and global conflicts emphasize the urgent need to secure food supplies while preserving ecosystems. There is a growing recognition that solutions must harmonize ecosystem restoration with providing high-quality nutrition for a rising global population. Seeds, particularly desiccation-tolerant seeds, have been crucial to the evolutionary success of terrestrial plants. The domestication of seed germination marked a pivotal event in the development of modern agriculture. However, in today's era of large-scale farming and climate change, selecting genotypes for germination traits has introduced new challenges. Issues such, low germination rates, seedling mortality, and preharvest sprouting—all linked to uncontrolled germination traits—pose critical threats to agriculture and ecological restoration efforts. Understanding how seeds regulate germination, influenced by both genetic and environmental factors, is essential for addressing these challenges. Seed dormancy, which prevents germination even under favorable conditions, is a key survival strategy but remains poorly understood at the molecular level. This project focused on deciphering the molecular mechanisms regulating seed dormancy, with a particular emphasis on the role of the DELAY OF GERMINATION 1 (DOG1) gene—a master regulator of dormancy. The goal was to explore how DOG1 interacts with abscisic acid (ABA) signaling to control this crucial process. By developing an innovative methodology that integrates molecular biology, biochemistry, proteomics, and a focused genetic strategy to unravel DOG1-mediated regulation of seed dormancy, this project has made significant strides in understanding DOG1 signaling. The key findings include: Unveiling the fundamental architecture of a dual molecular system in the control of ABA responses, with one pathway governed by DOG1 and the other by the classical ABA core signaling pathway. - Demonstrating that DOG1 is involved in the formation of phase-separated bodies. - Revealing the distinct roles of AHG1 and AHG3 in seed germination through proteomics.
Publications
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ABI5 binding protein2 inhibits ABA responses during germination without ABA-INSENSITIVE5 degradation. Plant Physiology, 189(2), 666-678.
Lynch, Tim; Née, Guillaume; Chu, Avan; Krüger, Thorben; Finkemeier, Iris & Finkelstein, Ruth R.
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“A watch DOG to prevent shut down of ABA responses during early seed imbibition.” August 2021 (13th Triennial Meeting of the ISSS; Brighton-UK)
Née, Guillaume
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“A watch DOG to prevent shut down of ABA responses during early seed imbibition.” January (Plant Seminar; Uni Geneva-SW)
Née, Guillaume
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“A watch DOG to prevent shut down of ABA responses during early seed imbibition.” March (SPS Seminar; IPS2 Paris-FR)
Née, Guillaume
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“A watch DOG to prevent shut down of ABA responses during early seed imbibition.” September (LGDP seminar; CNRS-Perpignan-FR).
Reichheld, Phillipe
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Dry side of the core: a meta-analysis addressing the original nature of the ABA signalosome at the onset of seed imbibition. Frontiers in Plant Science, 14.
Née, Guillaume & Krüger, Thorben
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“Genetic and molecular basis for the control of seed dormancy by DOG1 independently of the ABA core machinery.” June (CRAG seminar; CRAG-Barcelone-ES)
Née, Guillaume
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“How seeds adapt their germination time without impacting their stress-reactiveness” November (EPS Theme 3 Symposium “metabolism and adaptation”; Utrecht Universitiy-NL)
Née, Guillaume
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”How seeds adapt their germination time without impacting their stress-reactiveness” December (IPK Vavilov-Seminar; IPK-DE)
Née, Guillaume
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ABI5 binding proteins: key players in coordinating plant growth and development. Trends in Plant Science, 29(9), 1006-1017.
Vittozzi, Ylenia; Krüger, Thorben; Majee, Adity; Née, Guillaume & Wenkel, Stephan
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DOG1 prevents AFPs activation by AHG1 to control dormancy separately from ABA core signaling. openRxiv.
Krüger, Thorben; Brandt, Dennis; Sodenkamp, Johanna; Gasper, Michael; Romera-Branchat, Maida; Ahloumessou, Florian; Gehring, Elena; Drotleff, Julia; Bell, Christopher; Kramer, Katharina; Eirich, Jürgen; Soppe, Wim J. J.; Finkemeier, Iris & Née, Guillaume
