The effect of DNA modifications on the stability and mobility of nucleosomes (A03)

• Subject Area: Structural Biology
• Term: from 2018 to 2022
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 325871075

In addition to the canonical DNA bases chemically modified bases have been found recently. In particular for 5-fC a role in epigenetics is certain. How this modification affects gene expression is so-far elusive. On one hand, we will characterize potential changes in structure and dynamics of DNA double strands upon inclusion of 5-fC. On the other, changes in DNA-histone interactions in nucleosomes due to 5-fC will be determined. For these goals, we use NMR spectroscopy in solution and in the solid state.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1309: Chemical Biology of Epigenetic Modifications
• Applicant Institution: Ludwig-Maximilians-Universität München
• Project Heads: Professor Dr. Rasmus Linser, Ph.D.; Dr. Petra Rovó