Synthesis of membrane-permeable, enzymatically cleavable and photocaged second messenger and their role in cells (A04)

• Subject Area: Organic Molecular Chemistry - Synthesis and Characterisation
• Term: since 2018
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 335447717

The membrane-permeable derivatives of the second messengers NAADP, ADPR and cNMPs (MASTER-compounds) developed in the 2nd funding period will be further modified by new masking groups as well as photocleavable groups for spatial and temporal resolution of the biological processes. These biochemical tools will be used for cell-based studies to understand their biological role, e.g. NAADP/Ca2+ signaling in T cell aging. All-in-one-clickable derivatives will be prepared to identify intracellular location and expression of NAADP receptors. NAADP antagonists will be addressed as well.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1328: Adenine Nucleotides in Immunity and Inflammation
• Applicant Institution: Universität Hamburg
• Project Heads: Dr. Feng Gu, since 7/2026; Professor Dr. Chris Meier