Function and stimulation of the transient increase of the leaf apoplastic pH during exposure to chlorine-stress
Final Report Abstract
The research program tested three main hypotheses to understand the influence of anions on pHapo increase, the role of root pHapo in short-term salt stress response, and the link between pHapo increase and abscisic acid (ABA) accumulation in guard cells. The results show that not only chloride, but also nitrate, phosphate, and sulfate ions induce alkalinization of the leaf apoplast, but the magnitude and timing of pHapo responses vary based on the specific anion type and the developmental stage of the leaf. It was found that the addition of NaCl to the rooting medium did not cause any significant changes in root apoplastic pH. This suggests that root pHapo might not be a significant factor in the short-term stress response of Vicia faba plants. Furthermore, the study explored the impact of pHapo increase on stomatal closure and ABA synthesis. The results indicate that the pHapo transient alone can initiating stomatal closure and reduce stomatal conductance, independent of interactions with other chloride-stress-related responses. The pHapo-based de-novo synthesis of ABA in the leaves contributes to the reduction in stomatal conductance and transpiration rate. These findings are relevant as they shed light on how pH changes in the leaf apoplast play a crucial role in regulating stomatal closure and ABA synthesis. The research provides insights into the fundamental processes that govern plant responses to stress.
Publications
-
Leaf apoplastic alkalization promotes transcription of the ABA-synthesizing enzyme Vp14 and stomatal closure in Zea mays. Journal of Experimental Botany, 72(7), 2686-2695.
Geilfus, Christoph-Martin; Zhang, Xudong; Mithöfer, Axel; Burgel, Lisa; Bárdos, Gyöngyi & Zörb, Christian
-
Phenolic compound abundance in Pak choi leaves is controlled by salinity and dependent on pH of the leaf apoplast. Plant-Environment Interactions, 2(1), 36-44.
Meyer, Philipp; Förster, Nadja; Huyskens‐Keil, Susanne; Ulrichs, Christian & Geilfus, Christoph‐Martin
-
Why do plants blush when they are hungry?. New Phytologist, 239(2), 494-505.
Jezek, Mareike; Allan, Andrew C.; Jones, Jeffrey J. & Geilfus, Christoph‐Martin
