Strukturelle und funktionelle Analyse der Interaktion zwischen Kinetochor und zentromerischem Chromatin in Saccharomyces cerevisiae
Zellbiologie
Zusammenfassung der Projektergebnisse
We report an unanticipated role for enzymes required for b-1,6 and b-1,3 glucan biosynthesis in regulating kinetochore function in Saccharomyces cerevisiae. These carbohydrates are the major constituents of the yeast cell wall. Deletion of KRE6 - a glycosylhydrolase critical for b-1,6 glucan synthesis suppressed the defects caused by mutations in components of the kinetochore. Our extensive genetic analyses revealed that there was a trend in the suppression pattern in that several mutations of outer kinetochore components were strongly suppressed, whereas mutations of inner kinetochore components were either unaffected, and the growth defect of a temperature sensitive CSE4 allele was enhanced. We also found out that the absence of other genes involved in cell wall synthesis such as FKS1- b-1,3 glucan synthase, and KRE11- a TRAPPII component, suppressed the growth defects with the mutation in SPC25. Probably, the reduction of intracellular b-1,6 and b -1,3 glucan regulates kinetochore function. Interestingly, we found a physical interaction between Kre6 and Cse4 in yeast, suggesting a potential function for Kre6 in glycosylating Cse4 or another kinetochore protein. Altogether, our work suggests a potential role for carbohydrate synthesis factors and carbohydrate modification of kinetochore proteins in chromosome biology.
Projektbezogene Publikationen (Auswahl)
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A role for β-1,6- and β-1,3-glucans in kinetochore function in Saccharomyces cerevisiae. GENETICS, 226(2).
Kshirsagar, Rucha; Munhoven, Arno; Tran Nguyen, Tra My & Ehrenhofer-Murray, Ann E.
