Biochemical and electrochemical characterization of redox protein candidates of the iron oxidation in the acidophilic iron oxidizer “Ferrovum” sp. PN-J47-F6
Final Report Abstract
Iron oxidizing bacteria drive their metabolic processes by oxidizing ferrous iron to ferric iron and converting the energy of the electron transfer towards a terminal electron acceptor. Energy conversion thereby refers to the generation of ATP and a contribution to the proton motive force. Therefore, the microorganisms require a set of redox proteins that transfer electrons of the cellular surface to the cytoplasmic site of the inner membrane. The present project focused on the study of four redox proteins of the acidophilic iron oxidizer Ferrovum sp. PN-J47-F6 in order to evaluate a previously predicted model of the iron oxidation and to understand potential adaptational mechanisms to the acidic habitat of Ferrovum spp.. The latter aspect was intended to test the hypothesis of the evolutionary origin of the iron oxidation electron transfer of Ferrovum spp. in neutrophilic iron oxidizing bacteria.
Publications
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Shedding light into the electron transfer chain of a moderately acidophilic iron oxidizer: characterization of recombinant redox proteins of Ferrovum sp. PN-J47-F6. IBS 2022, Perth, Australia
Ullrich S.R., Fuchs H., Ritter A.-M. & Schlömann S.
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Electrochemical characterization of recombinant respiratory proteins of the acidophilic iron oxidizer Ferrovum sp. PN- J47-F6. VAAM 2023, Göttingen, Germany
Ullrich S.R., Fuchs H., Ashworth-Güth C. & Schlömann S.
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Heterologous production of c-type cytochromes in Vibrio natriegens. VAAM 2023, Göttingen, Germany.
Fuchs H., Ullrich S.R. & Hedrich S.
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Vibrio natriegens – a superior host for the heterologous production of redox proteins. FEMS 2023, Hamburg, Germany
Fuchs H., Hedrich S. & Ullrich S.R.
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Electrochemical and structural characterization of recombinant respiratory proteins of the acidophilic iron oxidizer Ferrovum sp. PN-J47-F6 suggests adaptations to the acidic pH at protein level. Frontiers in Microbiology, 15.
Ullrich, Sophie R.; Fuchs, Helena & Ashworth-Güth, Charlotte
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Shedding light on the electron transfer chain of a moderately acidophilic iron oxidizer: characterization of recombinant HiPIP-41, CytC-18 and CytC-78 derived from Ferrovum sp. PN-J47-F6. Research in Microbiology, 175(1-2), 104088.
Ullrich, Sophie R.; Fuchs, Helena & Schlömann, Michael
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Vibrio natriegens as a superior host for the production of c-type cytochromes and difficult-to-express redox proteins. Scientific Reports, 14(1).
Fuchs, Helena; Ullrich, Sophie R. & Hedrich, Sabrina
