Dissecting the response of replisomes to genotoxic stress (06)

• Subject Area: General Genetics and Functional Genome Biology
• Term: since 2019
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 393547839

This project aims at elucidating how the DNA replication machinery reacts to DNA lesions. We have gener-ated a large collection of engineered human and mouse cells with labelled components of the replication machinery as well as sensors for ubiquitin modifications in live cells. Using these models and a combination of microscopical and genomics approaches, we will generate functional and kinetic spatio-temporal models of the response of the genome replication machinery to damages and how cellular physiology is affected.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1361: Regulation of DNA Repair and Genome Stability
• Applicant Institution: Johannes Gutenberg-Universität Mainz
• Project Head: Professorin Dr. Maria Cristina Cardoso