Regulation of paternal inheritance of an Argonaute protein in C. elegans
Biochemie
Entwicklungsbiologie
Zusammenfassung der Projektergebnisse
In this project we have identified and dissected a molecular mechanism that can mediate the inheritance of gene regulation from father to offspring, and that this mechanism is important for the proper development of the germ cells in the offspring. Similar mechanisms may act in different processes that rely on subcellular organization and/or transport of molecules through a cell. The project developed roughly as planned, although we dedicated a lot of time to the PEI granule components PEI-2, which was not yet identified at the time of grant application. The interaction of the PEI granules with the FB-MOs was initially a surprise, but after learning more about these organelles, they in fact are THE vesicle you would expect to play a role in the segregation of molecules into spermatids. Whether the identified mechanism operates in humans is still unclear. However, we did identify potential homologs of the PEI proteins, and these are in fact expressed during spermatogenesis. Therefore, it seems reasonable to expect similar processes in human cells. Whether this knowledge can be applied is presently not an avenue that we have pursued. This work was picked up by the local press, and a small article appeared in the newspaper “Algemeine Zeitung-Rheinland-Pfalz”.
Projektbezogene Publikationen (Auswahl)
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Protease-mediated processing of Argonaute proteins controls small RNA association. Molecular Cell, 81(11), 2388-2402.e8.
Gudipati, Rajani Kanth; Braun, Kathrin; Gypas, Foivos; Hess, Daniel; Schreier, Jan; Carl, Sarah H.; Ketting, René F. & Großhans, Helge
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How stress can affect your sex appeal. Developmental Cell, 57(3), 291-292.
Schreier, Jan & Ketting, René F.
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Membrane-associated cytoplasmic granules carrying the Argonaute protein WAGO-3 enable paternal epigenetic inheritance in Caenorhabditis elegans. Nature Cell Biology, 24(2), 217-229.
Schreier, Jan; Dietz, Sabrina; Boermel, Mandy; Oorschot, Viola; Seistrup, Ann-Sophie; de Jesus Domingues Antonio, M.; Bronkhorst, Alfred W.; Nguyen, Dieu An H.; Phillis, Stephanie; Gleason, Elizabeth J.; L.’Hernault, Steven W.; Phillips, Carolyn M.; Butter, Falk & Ketting, René F.
