Regulation of plant chromatin organization by lamin proteins
Plant Genetics and Genomics
Final Report Abstract
In eukaryotes, genomic DNA in interphase nuclei shows non-random distribution patterns at the nuclear periphery, which is realized by constituent members forming the nuclear lamina. Functional characterization of plant nuclear lamins concerning chromatin organization is still in its infant stage. In this project, we have revealed fundamental characteristics of plant nuclear lamins in two areas. Firstly, we found considerable function diversification among lamins in evolutionarily separated plant species, that the sole lamin in Marchantia, i.e., MpNMCP, could not directly interact with chromatin. Secondly, we discovered and characterized the detachment of Arabidopsis lamina-associated chromatin domains in response to heat stress. Surprisingly, we also found that the Arabidopsis nuclear lamina disassembles under other abiotic stress conditions; after disassembly, individual nuclear lamina components largely maintain their physical interactions with chromatin and potentially shape the three-dimensional chromatin organization during stress adaptation. Such flexibility of the plant nuclear lamina under abiotic stress has not been described elsewhere; it likely represents a new exciting research area of three-dimensional plant genomics. Overall, this project has made significant scientific discoveries that advance our understanding of the chromatin-lamina network in plants, and will be of broad interest to researchers studying genome architecture, chromatin folding, and transcriptional regulation.
Publications
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Marchantia TCP transcription factor activity correlates with three-dimensional chromatin structure. Nature Plants, 6(10), 1250-1261.
Karaaslan, Ezgi Süheyla; Wang, Nan; Faiß, Natalie; Liang, Yuyu; Montgomery, Sean A.; Laubinger, Sascha; Berendzen, Kenneth Wayne; Berger, Frédéric; Breuninger, Holger & Liu, Chang
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Characterization of a Plant Nuclear Matrix Constituent Protein in Liverwort. Frontiers in Plant Science, 12 (2021, 5, 7).
Wang, Nan; Karaaslan, Ezgi Süheyla; Faiss, Natalie; Berendzen, Kenneth Wayne & Liu, Chang
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Spatial Features and Functional Implications of Plant 3D Genome Organization. Annual Review of Plant Biology, 73(1), 173-200.
Domb, Katherine; Wang, Nan; Hummel, Guillaume & Liu, Chang
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The plant nuclear lamina disassembles to regulate genome folding in stress conditions. (2022, 12, 2). American Geophysical Union (AGU).
Wang, Nan; Wang, Zhidan; Tzourtzou, Sofia; Wang, Xu; Bi, Xiuli; Leimeister, Julia; Xu, Linhao; Sakamoto, Takuya; Matsunaga, Sachihiro; Schaller, Andreas; Jiang, Hua & Liu, Chang
