Development of electrophoretic co-deposition of bioactive and antibacterial ceramics with biodegradable polymers to produce novel composite coatings for biomedical applications

• Applicant: Professor Dr.-Ing. Aldo Boccaccini
• Subject Area: Biomaterials
• Term: from 2019 to 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 426494347

• Final Report Year: 2024

Final Report Abstract

The deposition of novel biodegradable coatings with tailored bioactivity and antibacterial activity on metallic biomaterials for applications as dental and orthopedic implants is of high medical relevance in order to minimize the risk of complications after implantation and to limit the use of antibiotics. One of the most widely used metals in the dental and orthopedic fields is commercially pure titanium (CP-Ti) due to its high corrosion resistance and biocompatibility. In recent years metastable-β and β Ti alloys, such as Ti-13Nb-13Zr are also gaining increasing attention for biomedical applications due to their mechanical properties close to bone and their non-toxic behaviour in contact with the human body. However, Ti and its alloys exhibit bioinert properties and their osteoinductive capabilities have to be improved, e.g., by applying bioactive and biodegradable coatings on their surface. Among different coating techniques, electrophoretic deposition (EPD) can be used with a great variety of materials to deposit films on different three-dimensional substrates at room temperature. The coating microstructure and thickness can be controlled by varying the chemical composition of the suspension, the applied potential and the deposition time. Thus, the current project focuses on developing composite polymerbased coatings, incorporating various antibacterial agents on CP Ti and near-β Ti-13Nb-13Zr alloy using EPD. Zein, chitosan and alginate were selected as coating matrices, while different sol-gel derived bioactive glass particles or nanocrystalline hydroxyapatite (HA) and/or silicon nitride (Si3N4) and/or graphene oxide (GO) and/or copper oxide (CuO) were added as inorganic antibacterial agents in the coatings. The results of the project contribute to the development of novel multicomponent coatings by systematic investigation of key EPD parameters, including the suspension stability, the electrokinetic properties of particles in different electrolytes and the deposition mechanisms. Moreover, new knowledge was generated on the relation between the EPD process and selected properties of the final coatings, such as adhesion strength, micro-mechanical performance and biological activity. The obtained coatings can be considered as potentially suitable candidate materials for orthopedic and dental applications.

Publications

• Electrophoretic deposition and characterization of HA/sodium alginate coatings on commercially pure titanium, UK-Poland Conference on Bioinspired Materials, 23-24.11.2020, MS Teams, oral presentation
  M. Warcaba
  
• Innowacje w Metalurgii i Inżynierii Materiałowej, 12.05-13.05.2022, Kraków - referat na zaproszenie pt. Osadzanie elektroforetyczne jako wszechstronna metoda wytwarzania powłok kompozytowych na podłożach metalowych, invited lecture
  T. Moskalewicz
  
• Adhesion, microstructure and Surface topography of mesoporous sol-gel glass/zein coatings electrophoretically deposited on Ti-13Nb-13Zr alloy substrates, European Congress And Exhibition On Advanced Materials And Processes - EUROMAT 2021, poster
  F. Maciąg
  
• Electrophoretic deposition and characterization of CuO/GtO/HA/SA coatings on titanium alloy, 30th Anniversary Conference „Biomaterials in Medicine and Veterinary Medicine”, Rytro, Poland, 2021, poster
  M. Warcaba
  
• Electrophoretic deposition of zein/bioactive glass composite coatings doped with F and Cu on titanium for biomedical applications, 6th Euro BioMAT 2021 - European Symposium on Biomaterials and Related Areas 05 May 2021 - 06 May 2021, online, poster
  Z. Hadzhieva
  
• Electrophoretic deposition, microstructure and properties of multicomponent sodium alginate based coatings on titanium biomaterials, Materials Science & Technology 2021: Where Materials Innovation Happens, 17-20 October 2021, Columbus, Ohio, USA, oral presentation
  T. Moskalewicz
  
• Hydroxyapatite/sodium alginate coatings electrophoretically deposited on titanium substrates: microstructure and properties. Applied Surface Science, 540, 148353.
  Moskalewicz, Tomasz; Warcaba, Maciej; Cieniek, Łukasz; Sitarz, Maciej; Gajewska, Marta & Boccaccini, Aldo R.
  
• Impact of Surface Topography, Chemistry and Properties on the Adhesion of Sodium Alginate Coatings Electrophoretically Deposited on Titanium Biomaterials. Metallurgical and Materials Transactions A, 52(10), 4454-4467.
  Warcaba, Maciej; Kowalski, Kazimierz; Kopia, Agnieszka & Moskalewicz, Tomasz
  
• The Effect of Electrophoretic Deposition Parameters on the Microstructure and Adhesion of Zein Coatings to Titanium Substrates. Materials, 14(2), 312.
  Maciąg, Filip; Moskalewicz, Tomasz; Kowalski, Kazimierz; Łukaszczyk, Alicja; Hadzhieva, Zoya & Boccaccini, Aldo Roberto
  
• 49–ta Szkoła Inżynierii Materiałowej SIM, 29.09-30.09.2022, Kraków - referat na zaproszenie pt. "Osadzanie elektroforetyczne powłok polimerowych i kompozytowych o osnowie polimerowej na podłożach metalowych„ invited lecture
  T. Moskalewicz
  
• Bioactive glass containing antibacterial and bioactive coatings on chemically pre-treated titanium by electrophoretic deposition (EPD), 26th International Congress on Glass (ICG), 3-8 July 2022, Berlin, Germany, poster
  Z. Hadzhieva
  
• Development and characterization of zein-based coatings incorporating fluoride- and copper-doped bioactive glass on titanium for biomedical applications, Joint KMM-VIN / ViCEM / ESB cross-disciplinary workshop 22-23 September 2022, Vienna, Austria, oral presentation
  Z. Hadzhieva
  
• Development and Investigation of Mesoporous Bioactive Glass/Zein Coatings Electrodeposited on Titanium Alloy for Biomedical Applications. Metallurgical and Materials Transactions A, 54(1), 241-260.
  Maciąg, F.; Moskalewicz, T.; Cholewa-Kowalska, K.; Hadzhieva, Z.; Dziadek, M.; Łukaszczyk, A. & Boccaccini, A. R.
  
• Electrophoretic deposition of zein/bioactive glass composite coatings doped with F and Cu on titanium for biomedical applications, 7th International Conference on Electrophoretic Deposition: Fundamentals and Applications (EPD VII), Oct. 30 – Nov. 3, 2022 Santa Fe, USA, poster and oral presentation
  Z. Hadzhieva
  
• Electrophoretic deposition, microstructure and biological properties of mesoporous sol-gel glass/zein coatings on Ti-13Nb-13Zr alloy, 31st Annual Conference Biomaterials in Medicine and Veterinary Medicine, Rytro, Poland, 2022, oral presentation
  F. Maciąg
  
• Electrophoretic deposition, microstructure and properties of multicomponent sodium alginate-based coatings incorporated with graphite oxide and hydroxyapatite on titanium biomaterial substrates. Applied Surface Science, 575, 151688.
  Moskalewicz, Tomasz; Warcaba, Maciej; Łukaszczyk, Alicja; Kot, Marcin; Kopia, Agnieszka; Hadzhieva, Zoya & Boccaccini, Aldo R.
  
• EPD and characterisation of graphite oxide/hydroxyapatite/sodium alginate coatings incorporated with Si3N4 or CuO nanoparticles on titanium biomaterials, 7th International Conference on Electrophoretic Deposition: Fundamentals and Applications (EPD VII), Oct. 30 – Nov. 3, 2022 Santa Fe, USA, keynote
  T. Moskalewicz
  
• Fabrication and characterization of multicomponent coatings on titanium for biomedical applications, Materials Science and Engineering (MSE) Congress, 27- 29 September 2022, Darmstadt, Germany, poster
  Z. Hadzhieva
  
• Influence of Si 3 N 4 and CuO Nanoparticles on the Microstructure and Properties of Multiphase Graphite Oxide/Hydroxyapatite/Sodium Alginate Coatings Obtained By Electrophoretic Deposition on Titanium Alloy. Journal of The Electrochemical Society, 169(9), 092524.
  Moskalewicz, Tomasz; Warcaba, Maciej; Hadzhieva, Zoya; Łukaszczyk, Alicja; Jeleń, Piotr; Sitarz, Maciej & Boccaccini, Aldo R.
  
• Recent developments in electrophoretic deposition (EPD) of antibacterial coatings for biomedical applications - A review. Current Opinion in Biomedical Engineering, 21, 100367.
  Hadzhieva, Zoya & Boccaccini, Aldo R.
  
• Bioactive and antibacterial soft coatings by electrophoretic deposition, Workshop “Implantate – Wenn die Antwort in der Schicht steckt!” by the European Society of Thin Films e.V. (EFDS), 27-28 February 2023, Tuttlingen, Germany
  A.R. Boccaccini
  
• Biomaterial strategies to combat implant infections: new perspectives to old challenges. International Materials Reviews, 68(8), 1011-1049.
  Braem, Annabel; Kamarudin, Nur Hidayatul Nazirah; Bhaskar, Nitu; Hadzhieva, Zoya; Mele, Andrea; Soulié, Jérémy; Linklater, Denver P.; Bonilla-Gameros, Linda; Boccaccini, Aldo R.; Roy, Ipsita; Drouet, Christophe; Ivanova, Elena P.; Mantovani, Diego & Basu, Bikramjit
  
• Electrophoretic deposition (EPD) of zein/bioactive glass composite coatings doped with F and Cu on titanium for biomedical applications. Materials Letters, 351, 135066.
  Hadzhieva, Zoya; Cholewa-Kowalska, Katarzyna; Dlouhy, Ivo; Moskalewicz, Tomasz & Boccaccini, Aldo R.
  
• Electrophoretic deposition of zein-based coatings on titanium for biomedical applications, 7th European Symposium on Biomaterials and Related Areas (BioMAT), 3-4 May 2023, Weimer, Germany, oral presentation
  Z. Hadzhieva
  
• Influence of Mesoporous Bioactive Glass Particles Doped with Cu and Mg on the Microstructure and Properties of Zein-Based Coatings Obtained by Electrophoretic Deposition. Journal of The Electrochemical Society, 170(8), 082501.
  Maciąg, F.; Moskalewicz, T.; Cholewa-Kowalska, K.; Hadzhieva, Z.; Dziadek, M.; Dubiel, B.; Łukaszczyk, A. & Boccaccini, A. R.
  
• Sodium-free fluoride- and copper-doped bioactive glass containing zein coatings for biomedical applications, XVIII Conference & Exhibition of the European Ceramic Society, 2-6 July 2023, Lyon, France, oral presentation
  Z. Hadzhieva