Alternative splicing as an element of signal transduction in multi-step phosphorelay systems of fungi
Final Report Abstract
In the project "Alternative splicing as an element of signal transduction in multi-step phosphorelay systems of fungi", research was conducted on how molecular signal diversity in organisms comes about, using the example of the osmoregulation system of the filamentous phytopathogenic fungus Magnaporthe oryzae. More specifically, how in the signal transduction pathway responsible for this, the High Osmolarity Glycerol (HOG) signalling pathway, different (environmental) signals can be received as "input" from several sensor proteins and independently passed on as different "output" signals to different targets in the cell by only one phosphotransfer protein MoYpd1p. Using a combination of several bioinformatic tools, we were first able to identify 5 new isoforms based on transcriptome data and also validate them biologically. In addition, it was shown that M. oryzae produces at least three of these different isoforms of MoYpd1p in a signalspecific manner, which have different physiological functions. After different stressors, as they also occur equivalently in the plant, only certain MoYpd1p isoforms are produced in each case. After salt stress, for example, only isoforms T0 and T2 are produced, whereas after sorbitol stress, isoform T1 is increasingly produced. Furthermore, the importance of the interplay of all these isoforms in complex physiological processes such as virulence was investigated and presented. The isoforms can only together enable the pathogenic fungus to successfully colonise its host; mutant strains that only produce individual isoforms are less virulent.
Publications
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Evidence of a New MoYpd1p Phosphotransferase Isoform in the Multistep Phosphorelay System of Magnaporthe oryzae. Journal of Fungi, 7(5), 389.
Bühring, Sri; Yemelin, Alexander; Michna, Thomas; Tenzer, Stefan & Jacob, Stefan
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Quantitative Proteome and Phosphoproteome Profiling in Magnaporthe oryzae. Methods in Molecular Biology, 109-119. Springer US.
Michna, Thomas & Tenzer, Stefan
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Data-Independent Acquisition (DIA) Is Superior for High Precision Phospho-Peptide Quantification in Magnaporthe oryzae. Journal of Fungi, 9(1), 63.
Bersching, Katharina; Michna, Thomas; Tenzer, Stefan & Jacob, Stefan
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Recent Advances in Research on Molecular Mechanisms of Fungal Signaling. Encyclopedia, 2(2), 840-863.
Jacob, Stefan; Bühring, Sri & Bersching, Katharina
