Octopamine function revisited: Reward processing in Drosophila larvae
Final Report Abstract
Octopamine and tyramine are known to impact on various aspects of an insect´s life. They function as neurotransmitters/-hormones and shift the organism from a relaxed state to a responsive, exited, and alerted state, which appears functionally homologous to the noradrenergic/adrenergic system of vertebrates. To do so, released octopamine/tyramine modulate muscle performance, glycogenolysis, fat metabolism, heart rate, and respiration. In addition, (in particular) octopamine also appears to fundamentally impact on learning and memory. About 20 years ago, initial work demonstrated that dopaminergic neurons signal punishment and octopaminergic neurons signal reward during olfactory associative conditioning in Drosophila. In the meantime, however, it has become clear that dopaminergic neurons mediate both reward and punishment signals to the mushroom bodies. This seems to somehow overturn the previous functionally separated model and limits the function of octopaminergic neurons as teaching neurons during conditioning. We therefore focused on a detailed description of octopamine in larval memory processes. By a synergistic use of behavioural studies, functional Ca2+ imaging, loss-of-function (RNA interference of specific receptors) and gain-of-function (optogenetics) approaches we show that octopamine is necessary for larval odour-reward memories through the specific modulation of dopaminergic neurons of the pPAM cluster.
Publications
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Antinociceptive modulation by the adhesion GPCR CIRL promotes mechanosensory signal discrimination. eLife, 9.
Dannhäuser, Sven; Lux, Thomas J.; Hu, Chun; Selcho, Mareike; Chen, Jeremy T.-C.; Ehmann, Nadine; Sachidanandan, Divya; Stopp, Sarah; Pauls, Dennis; Pawlak, Matthias; Langenhan, Tobias; Soba, Peter; Rittner, Heike L. & Kittel, Robert J.
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Food restriction reconfigures naïve and learned choice behavior inDrosophilalarvae. Journal of Neurogenetics, 34(1), 123-132.
Brünner, Benita; Saumweber, Juliane; Samur, Merve; Weber, Denise; Schumann, Isabell; Mahishi, Deepthi; Rohwedder, Astrid & Thum, Andreas S.
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Optogenetics: Illuminating neuronal circuits of memory formation. Journal of Neurogenetics, 34(1), 47-54.
Ehmann, Nadine & Pauls, Dennis
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Endocrine signals fine-tune daily activity patterns in Drosophila. Current Biology, 31(18), 4076-4087.e5.
Pauls, Dennis; Selcho, Mareike; Räderscheidt, Johanna; Amatobi, Kelechi M.; Fekete, Agnes; Krischke, Markus; Hermann-Luibl, Christiane; Ozbek-Unal, Ayten Gizem; Ehmann, Nadine; Itskov, Pavel M.; Kittel, Robert J.; Helfrich-Förster, Charlotte; Kühnlein, Ronald P.; Mueller, Martin J. & Wegener, Christian
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Learning and Memory inDrosophilaLarvae. Cold Spring Harbor Protocols, 2023(3), pdb.top107863.
Weber, Denise; Richter, Vincent; Rohwedder, Astrid; Großjohann, Alexandra & Thum, Andreas S.
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The Analysis of Aversive Olfactory–Taste Learning and Memory inDrosophilaLarvae. Cold Spring Harbor Protocols, 2023(3), pdb.prot108050.
Weber, Denise; Richter, Vincent; Rohwedder, Astrid; Großjohann, Alexandra & Thum, Andreas S.
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Neuronal correlates of time integration into memories.
Frantzmann, Felix; Lamberty, Marius; Braune, Laurin; Auger, Genevieve M.; Chouhan, Nitin Singh; Langenhan, Tobias; Selcho, Mareike & Pauls, Dennis
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Rewarding Capacity of Optogenetically Activating a Giant GABAergic Central-Brain Interneuron in Larval Drosophila. The Journal of Neuroscience, 43(44), 7393-7428.
Mancini, Nino; Thoener, Juliane; Tafani, Esmeralda; Pauls, Dennis; Mayseless, Oded; Strauch, Martin; Eichler, Katharina; Champion, Andrew; Kobler, Oliver; Weber, Denise; Sen, Edanur; Weiglein, Aliće; Hartenstein, Volker; Chytoudis-Peroudis, Charalampos-Chrysovalantis; Jovanic, Tihana; Thum, Andreas S.; Rohwedder, Astrid; Schleyer, Michael & Gerber, Bertram
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Four individually identified paired dopamine neurons signal taste punishment in larval Drosophila. eLife, 12.
Weber, Denise; Vogt, Katrin; Miroschnikow, Anton; Pankratz, Michael J. & Thum, Andreas S.
