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Projekt Druckansicht

Kombinierte Bildgebung basierend auf kohärenter Raman-Streuung und Fluoreszenz zur Untersuchung von Zuckeraufnahme in lebenden Zellen

Antragstellerin Dr. Evelyn Plötz
Fachliche Zuordnung Biophysik
Analytische Chemie
Förderung Förderung von 2019 bis 2024
Projektkennung Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 426814631
 
Erstellungsjahr 2024

Zusammenfassung der Projektergebnisse

Understanding the roles of sugars in cellular processes is crucial due to their importance in metabolic pathways and glycosylation processes. Glycans, built up from single monosaccharides, impact processes like cell signaling, adhesion, and form the glycocalyx. Changes in glycosylation often relate to pathological conditions, such as inflammation and tumorigenesis, highlighting the need to understand sugar composition and function. While structural approaches provide detailed glycome analysis, they lack spatial information about glycan distribution within cells. Optical microscopy, especially fluorescence imaging, offers insights into subcellular structures and dynamic processes. Metabolic oligosaccharide engineering allows for sugar labeling in cells. Yet, the incorporation path of unnatural sugars into cells remains unclear, necessitating innovative imaging approaches. We aimed to implement stimulated Raman scattering (SRS) microscopy, combined with fluorescence-based live-cell imaging, to study GLUT transporter mediated sugar uptake and storage in cells. Good progress was made in developing an SRS microscope, combining it with confocal fluorescence microscopy, and developing acquisition software for correlative, multimodal microscopy. The established MOSAIC microscopy setup allows for correlative multi-color fluorescence imaging, nonlinear microscopy and space-resolved Raman spectroscopy for in-situ measurements. We benchmarked the imaging system for transparent, solid state samples like graphene, and optimized it for soft-matter imaging through projects involving tissue sections, spheroids and single cells, that helped us to expand MOSAIC even to Fluorescence-Lifetime microscopy. As cellular sugar uptake is tightly regulated, we lastly explored metal-organic-framework (MOF) as carriers for sugars and studied various MOFs at the single-particle level. Our research uncovered new material properties and their significant impact on parameters, such as optical response or uptake capacities for guest-molecules. We used MOSAIC to investigate ‘small’ molecules in MOFs employed for atmospheric water harvesting, hydrogen storage and CO2 capture. Moving forward, we currently develop advanced microscopy tools to track MOF nanoparticle dynamics in within cellular environments.

Projektbezogene Publikationen (Auswahl)

 
 

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