Development of an Automated On-line Hyphenation of Capillary Electrophoresis with Multicollector ICP-MS for Species-specific Isotopic Analysis of Biomolecules
Final Report Abstract
Methods for analyzing isotope ratios are becoming increasingly popular, especially in the lifesciences, as they provide detailed insights into the speciation of elements and processes at the molecular level. Inductively coupled plasma-mass spectrometry (ICP-MS) provides valuable information here, as it enables the precise analysis of isotope ratios at trace level concentration. In combination with chromatographic or electrokinetic separation methods, ICP-MS becomes even more powerful and enables efficient and species-specific isotope analysis, especially for complex biological samples. Capillary electrophoresis (CE) as a high-resolution separation method is particularly suitable for biological matrices, as it requires minimal sample quantities and at the same time high separation power. Sulphur is a central element in proteins and enzymes, which play a key role in amino acid synthesis and cell metabolism. The analysis of sulfur isotope ratios in different elemental species provides valuable insights in different areas - e.g., metabolic pathways, disease mechanisms or environmental processes. However, current sulfur analysis methods mostly perform bulk sulfur analyses (concentrations, isotopes), which limits the ability to distinguish between individual sulfur-containing species. However, this is particularly relevant for biological samples in which sulfur occurs in different forms, each of which fulfills different biochemical functions (e.g., different proteins). It is therefore essential to separate the sulfur species before analysis. The on-line CE/ICP-MS method offers a promising approach, but is associated with various challenges, such as the coupling of the analytical devices, the optimization of the parameters of the analytical method (especially regarding isotope ratios) and the handling of complex samples. The focus of the research project was therefore on the further development of CE/ICP-MS as a robust on-line analysis platform for species-specific isotope analyses.
Publications
-
Coupling of Capillary Electrophoresis with multicolletor ICP-MS for species-specific isotope analysis of sulfur, 6th Doctoral Student Seminar of the German Working Group for Analytical Spectroscopy (DAAS), 08.2021, Online, Siegen, Germany
D. Tukhmetova, S. Faßbender & B. Meermann
-
Towards on-line species-specific isotopic analysis of sulfur in biomolecules, BAM Themenfeld-Tag "Analytical Sciences", 03.2021, Berlin, Germany
D. Tukhmetova, S. Faßbender & B. Meermann
-
Data processing made easy: standalone tool for automated calculation of isotope ratio from transient signals – IsoCor. Journal of Analytical Atomic Spectrometry, 37(11), 2401-2409.
Tukhmetova, Dariya; Lisec, Jan; Vogl, Jochen & Meermann, Björn
-
Data processing tool for automated calculation of isotope ratios from transient signals – IsoCor, 28th ICP-MS Users Meeting, 09.2022, Leoben, Austria
D. Tukhmetova, J. Lisec, J. Vogl & B. Meermann
-
On-line species-specific isotope analysis of sulfur in biomolecules via CE/MC-ICP-MS, European Winter Conference on Plasma Spectrochemistry, 01.2023, Ljubljana, Slovenia
D. Tukhmetova, J. Lisec, J. Vogl & B. Meermann
-
On-line Species-specific Isotopic Analysis of Sulfur by Hyphenation of Capillary Electrophoresis with Multicollector-ICP-MS, European Winter Conference on Plasma Spectrochemistry, 01.2023, Ljubljana, Slovenia
D. Tukhmetova, S. Faßbender & B. Meermann
-
Quantification of sulfur containing biological compounds via coupling CE with on-line Isotope Dilution CP-MS. 51st International Symposium HPLC 2023, 06.2023, Duesseldorf, Germany.
D. Tukhmetova, J. Lisec, J. Vogl & B. Meermann
-
Separation techniques hyphenated with ICP-MS: Data processing tool for isotope ratio analysis – IsoCor, 33th Doctoral Student Seminar of the German Working Group for Separation Science, 01.2023, Hohenroda, Germany
D. Tukhmetova, J. Lisec, J. Vogl & B. Meermann
-
Comparative study of the vibrating capillary nebulizer (VCN) and commercially available interfaces for on-line coupling of capillary electrophoresis with ICP-MS. Analytical and Bioanalytical Chemistry, 416(7), 1613-1621.
Taylor, Tristen L.; Tukhmetova, Dariya; Duong, Thi Phuong Thanh; Böwe, Anna-Maria; Meermann, Björn & Gundlach-Graham, Alexander
-
Development of an Online Isotope Dilution CE/ICP–MS Method for the Quantification of Sulfur in Biological Compounds. Analytical Chemistry, 96(8), 3276-3283.
Tukhmetova, Dariya; Lisec, Jan; Vogl, Jochen & Meermann, Björn
-
Online Isotope Analysis of Sulfur in Proteins via Capillary Electrophoresis Coupled With Multicollector ICP‐MS (CE/MC‐ICP‐MS): A Proof of Concept Study. ELECTROPHORESIS, 46(5-6), 290-295.
Tukhmetova, Dariya; Langhammer, Nicole; Vogl, Jochen & Meermann, Björn
