MAP kinase regulation of PIN-FORMED auxin transporters
Final Report Abstract
The directed distribution of the hormone auxin through the so-called PIN transporters has a significant impact on plant growth. PIN transporters undergo phosphorylation and activation by the AGC protein kinase. In recent years, there has been evidence that PINs are also regulated by members of the MAP kinase family. It is remarkable that the phosphorylation sites of both kinase types lie directly next to each other within conserved motifs. In a systematic investigation, we were able to demonstrate that both types of phosphorylation exist at the cellular plasma membrane, that phosphorylation is not dependent on individual protein kinases, and that it is necessary for PIN function. The inactivation of the phosphorylation sites leads to a changed protein distribution at the plasma membrane on a cellular level. With this, we have analyzed a new regulatory mechanism of the PINs that will contribute to the structural and biological understanding of PIN function.
Publications
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Editorial overview: Cell biology and cell signalling. Current Opinion in Plant Biology, 70, 102312.
Sprunck, Stefanie; Schwechheimer, Claus & Morita, Miyo T.
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Phosphorylation control of PIN auxin transporters. Current Opinion in Plant Biology, 65, 102146.
Bassukas, A.E. Lanassa; Xiao, Yao & Schwechheimer, Claus
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D6PK plasma membrane polarity requires a repeated CXX(X)P motif and PDK1-dependent phosphorylation. Nature Plants, 10(2), 300-314.
Graf, Alina; Bassukas, Alkistis Eleftheria Lanassa; Xiao, Yao; Barbosa, Inês C. R.; Mergner, Julia; Grill, Peter; Michalke, Bernhard; Kuster, Bernhard & Schwechheimer, Claus
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Signals and Signal Transduction. Fundamentals of Plant Physiology. Oxford University Press.
Taiz, Lincoln; Møller, Ian Max; Murphy, Angus & Peer, Wendy A.
