Project Details
Projekt
Nanoscale regulation of molecular switches at the Golgi (A20)
Subject Area
Biochemistry
Term
since 2020
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 278001972
Project A20 will investigate the mechanisms of regulation of ARF GTPases at the ER-Golgi interface. In light of their findings suggesting a differential role for ARF1 and ARF4 in ER-Golgi transport and organization, during the next funding period A20 will address how the two GTPases are recruited to different membrane nano-domains and activated by ARF GDP-GTP exchange factors (GEFs). They will employ CRISPR-Cas9 knock-in methodology in combination with proximity biotinylation using TurboID and mass spectrometric analysis, dynamic confocal and live-cell STED microscopy and perturbation via CRISPR-Cas9 knock outs of ARF GEFs.
DFG Programme
CRC/Transregios
Applicant Institution
Freie Universität Berlin
Project Head
Professorin Francesca Bottanelli, Ph.D.
