Project Details
Projekt Print View

Cell-type specific multiscale imaging of leukocytes through the combination of protein tagging and radiochemical labelling (A01)

Subject Area Biochemistry
Term since 2021
Project identifier Deutsche Forschungsgemeinschaft (DFG) - Project number 431460824
 
In vivo imaging of distinct leukocyte populations on multiple scales is vital for understanding their dynamics and roles in inflammation. To avoid premature label loss of ex vivo labelled cells, e.g., ER-HoxB8, we have devised self-labelling enzyme or nanobody (Nb) epitope tags to be genetically introduced as modular tagging platforms. These tags can be visualized in vivo days after cell differentiation and re-injection with label-customized substrates or Nbs. Focusing on radioactive labels as robust readouts in whole-body imaging, we plan to enhance the signal-to-background ratio by using improved and engineering covalently binding Nbs and by increasing their specific radioactivity through novel chemical separation methods.
DFG Programme Collaborative Research Centres
Applicant Institution Universität Münster
Project Heads Dr. Christian Paul Konken, since 1/2025; Professor Dr. Henning D. Mootz; Professorin Dr. Andrea Rentmeister, from 1/2024 until 12/2024
 
 

Additional Information

Textvergrößerung und Kontrastanpassung