Project Details
Projekt
Cell-type and activation-specific labelling of different leukocyte populations in vivo by bioorthogonal tagging (A01)
Subject Area
Biochemistry
Term
since 2021
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 431460824
Imaging of different leukocyte populations in vivo is key to understand their dynamics and role in inflammation. To overcome current limitations, we will develop new methods to label immune cells within a longer period of time, i.e., from inflammation initiation to resolution. We will establish a flexible and modular platform suitable for different modalities, such as fluorescence, scintigraphic or photoacoustic imaging. ER-HoxB8 cells will be genetically tagged ex vivo with self-labelling enzymes or epitopes for antibody fragments. After differentiation and re-injection, in vivo labelling will be achieved by administering tag-specific, functionalized labels to selectively visualize the respective cell population in a cutaneous granuloma mouse model.
DFG Programme
Collaborative Research Centres
Applicant Institution
Universität Münster
Project Heads
Professor Dr. Henning D. Mootz; Professorin Dr. Andrea Rentmeister, since 1/2024
