Applying the Concept of Synthetic Lethality to the Treatment of Autosomal Dominant Polycystic Kidney Disease (P05)

• Subject Area: Anatomy and Physiology ; Nephrology
• Term: since 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 431984000

P5 aims at identifying a novel therapeutic approach for polycystic kidney disease by applying the concept of synthetic lethality to the treatment of ADPKD. P5 developed a robust cell-based assay for high-throughput screening of synthetic lethality in ADPKD. Based on preliminary data from a chemical library screen of >50,000 compounds and a proposed genome-wide shRNA screen to identify genetic synthetic lethal interactions with Pkd1, P5 will validate identified promising compounds in cell-based 2D and 3D models of ADPKD, followed by testing in ADPKD mouse models.

• DFG Programme: Collaborative Research Centres
• Subproject of: SFB 1453: Nephrogenetics (NephGen)
• Applicant Institution: Albert-Ludwigs-Universität Freiburg
• Project Head: Professor Dr. Michael Köttgen