Project Details
Projekt
Time of Flight Mass Spectrometer (UHPLC-qToF-MS)
Subject Area
Biological Chemistry and Food Chemistry
Term
Funded in 2021
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 460221948
The instrument applied for is a time-of-flight mass spectrometer with upstream quadrupole and collision cell (qToF / UHR-TOF). The inlet systems are selectable between electrospray (ESI) ionization and atmospheric pressure chemical ionization (APCI) ionization for more polar components. The separation system shall be a high pressure HPLC system (UHPLC), which can also be used as an online SPE module. At the TU Kaiserslautern numerous projects have been carried out for years using tandem mass spectrometry ((U)HPLC-ESI/APCI-MS/MS). The working group Richling deals with the identification and quantification of food ingredients (focus on polyphenols and heat induced contaminants). Besides the occurrence of the above mentioned compound classes in food, their metabolism and biological effects are investigated in vitro, ex vivo and in humans. Within the framework of previous research projects, the analysis often required complex evaluation of the postulated compounds, e.g. of DNA adducts, also by means of the synthesis of a reference, if this was not commercially available (targeted approach). With the time-of-flight mass spectrometer applied for, biological samples were to be examined in the so-called non-targeted approach. This allows the additional identification of previously unknown compounds and the correlations of food intake with the occurrence of biomarkers such as biomarkers of exposure to contaminants such as furan or acrylamide or the correlation of the intake of biologically active compounds and their effects in the body. In the long term, the device applied for should make it possible to sensitively detect DNA or protein adducts of toxicologically relevant compounds or to correlate consumption habits via the analysis of urine samples. The co-applicants from the Departments of Food Chemistry and Toxicology (Fahrer group), Biology (Erkel and Frankenberg-Dinkel groups) and Mechanical and Process Engineering (Ulber group) would like to use the device within the scope of their research priorities, since no qToF device for the analysis of naturally occuring substances and their metabolites is available on the campus of the TU Kaiserslautern.
DFG Programme
Major Research Instrumentation
Major Instrumentation
Flugzeitmassenspektrometer (UHPLC-qToF-MS)
Instrumentation Group
1700 Massenspektrometer
Applicant Institution
Rheinland-Pfälzische Technische Universität Kaiserslautern-Landau
