Project Details
Projekt
Engineering conformational dynamics of a de novo designed two-domain metalloenzyme (A14#)
Subject Area
Biochemistry
Term
from 2021 to 2024
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 201302640
This project aims to turn a de novo designed metalloprotein into an artificial nuclease, in which the interplay of protein function and dynamics can be studied in an evolutionarily unbiased fashion. We plan to re-engineer the hydrophobic dimer interfaces of the two-domain scaffold as well as the domain-connecting flexible loops, such that binding of metal cofactor and substrate leads to conformational transitions and formation of a functional enzyme complex.
DFG Programme
Collaborative Research Centres
Applicant Institution
Technische Universität München (TUM)
Project Head
Professorin Dr. Cathleen Zeymer
