UHPLC coupled with a single quadrupole mass spectrometer for high throughput screening

• Subject Area: Molecular Chemistry
• Term: Funded in 2021
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 495518010

Requested is a uHPLC coupled to a mass spectrometer for high-throughput chromatographic screening of organic molecules and, in some cases, small peptides. The uHPLC system for rapid chromatographic separation is of key importance here. In particular, the uHPLC system shall be equipped with a multisampler that can apply samples very rapidly (<10s cycle time) from microtiter plates and enables analysis at very high pressure (>1000 bar). The mass spectrometer shall be equipped with electrospray ionization as ion source and single quadrupole as analyzer. In order to determine low concentrations of analytes in a high throughput, mass-selective, chromatographic manner, the LC/MS system is optimized for short cycle times, high sensitivity, large number of samples and high pressure. Given a positive response for this proposal, the LC/MS system will enable a variety of research projects at Bielefeld University. Scientifically, we aim to develop i) new methods in organic synthesis, ii) new non-natural enzyme functions, as well as new enzyme engineering methods.

• DFG Programme: Major Research Instrumentation
• Major Instrumentation: UHPLC gekoppelt mit Single-Quadrupol-Massenspektrometer für das Hochdurchsatz-Screening
• Instrumentation Group: 1700 Massenspektrometer
• Applicant Institution: Universität Bielefeld
• Leader: Professor Dr. Stephan C. Hammer