Project Details
Projekt
Genome-wide activity-based profiling of Arabidopsis proteomes
Subject Area
Plant Cell and Developmental Biology
Term
from 2007 to 2011
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 50069751
Information on protein activity is essential to the annotation and understanding of protein functions since it is the activity of a protein, rather than its abundance, that ultimately dictates its role in a living organism. We aim to uncover the proteome activity layer of Arabidopsis through a novel, genome-wide technology called activity-based proteome profiling (ABPP). ABPP can be applied on living tissues or on protein extracts and is based on labeled small molecules (probes) that react with active site residues of a broad range of proteins in an activity-dependent manner. Labeled proteins can be separated and visualized on protein gels and identified by mass spectrometry (MS). This project aims to generate activity information of 100-1000 different proteins from various mechanistic classes from different Arabidopsis proteomes through an intimate interdisciplinary collaboration between an organic chemist, biochemist and MS-specialist. Activities will be characterized and confirmed by heterologous expression. Labeling sites of as yet non-annotated proteins will be identified and validated to reveal their catalytic mechanisms. Finally, inhibitory signatures will be generated to sub-classify a large redundant protein family into functional units. Data will be shared through an open-access searchable database where plant scientists can retrieve activity information for their favorite protein(s) and request probes and protocols for further studies.
DFG Programme
Research Grants
