Project Details
Projekt
Form-function relationships in the development of Bacillus subtilis fruiting bodies
Applicants
Dr. Ilka Bischofs-Pfeifer; Professor Dr. Karl Rohr
Subject Area
Metabolism, Biochemistry and Genetics of Microorganisms
Term
since 2022
Project identifier
Deutsche Forschungsgemeinschaft (DFG) - Project number 504020040
Natural isolates of the endospore-forming model bacterium Bacillus subtilis show complex multicellular traits. Similar to plants, out of a spore of B. subtilis a 3D macroscopic multicellular structure can emerge that produces many more spores - a fruiting body. Different cell types partake in the formation of a fruiting body and its substructures. In this project, we will study how cell-type specific functions support the formation of biofilm wrinkles (“function drives form”) and how macrocolony architecture affects the production of spores (“form drives function”). With the help of cell-type specific fluorescent reporters we will image processes at the level of the entire macrocolony and at the level of its substructures down to individual cells (spores). We will develop computer-based image analysis methods based on deep learning in order to quantify cellular state transitions in space and time. With this approach – and other methods - we will test different hypotheses regarding the formation of fruiting bodies by targeted genetic manipulations of B. subtilis. This will reveal whether and how these genetic elements contribute to emergent properties during multicellular development.
DFG Programme
Priority Programmes
