Konfokales Laserscanning-Mikroskop
Zusammenfassung der Projektergebnisse
Center for Regenerative Medicine / Dept. Orthop. Surgery; PI: Aicher: - Investigation of stem cellniches: localisation of mesenchymal stromal cells (MSC) in human term placenta and investigation of cells and extra cellular matrix surounding MSC in placenta. - Investigation of attachment of MSC to components of the extracellular matrix (ECM) and ECM-derived peptides. These studies are designed to explore the influence of the ECM on differentiation of MSC and to design customized peptides for improved scaffo lds for tissue engineering. - Detection of distinct MSC subsets in perivascular sites. Center for Regenerative Medicine / Dept. Traumatology; PI: Rolauffs / Aicher: - Investigation of cellular localisation and three dimensional cellular patterns of chondrocytes in human cartilage in different stages of development, in health and d isease. Department of Anesthesiology and Intensive Care Medicine Center for Inflammation and Hypoxia; Pl: Köhler; Rosenberger (bis 2010); Eltzschig (bis 2008): - Regulation of Equilibrative Nucleoside Transporter (ENT) and Adenosine deaminase (ADA) in Hypoxia and Inflammation - Role of vasodilator-stimulated phosphoprotein (VASP), an actin cellsurface linking protein under hypoxic, ischemic and inflammatory conditions - Regulation and function of neuronal guidance molecules in inflammatory and ischemic processes Dept. of Internal Medicine II / Transplantation Immunology; PI: Kle in: - Analysis of the human hematopoietic stem cell niche role of cell adhesion molecules (special focus: integrin a9b role of extracellular matrix components (special focus: fibulin and laminin family) role of proteolytic enzymes (special focus: cathepsins) - Analysis of tumor cell interactions with the human bone marrow microenvironment - Analysis of T-cell development in the human thymus role of MMPs on thymic epithelial cells role of conduits, a specialized extracellular matrix structure role of perivascular spaces at the cortico-medullary junction Dept. of Internal Medicine II / Transplantation Immuno logy; PI: Pawelek: - Investigation of heat sho ck protein (HSP) expression in melanoma tissue. HSPs play an important role in cancer progression and are up-regulated in a variety of cancer types. In this study the expression of hsp90, hsp70, hsp40 and hsp32 were investigated. - Comparison of lipid rafts in primary vs. metastatic melanoma cell lines. - Expression of cell surface markers on T cells after a ctivation by anti-CD3-CD28-coup led beads.
Projektbezogene Publikationen (Auswahl)
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HIF-1-dependent repression of adenosine kinase attenuates hypoxia-induced vascular leak. Blood. 2008 Jun 5;111(12):5571-80
Morote-Garcia JC, Rosenberger P, Kuhlicke J, Eltzschig HK
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Neutrophil-facilitated platelet translocation identifies functional expression of epithelial ecto-nucleoside triphosphate diphosphohydrolase (NTPDase) activity. Journal of Clinical Investigation 2008 Nov;118(11):3682-92
Weissmuller T, Campbell EL, Rosenberger P, Scully M, Furuta G, Colgan SP
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Oxygen-independent stabilization of hypoxia inducible factor (HIF)-1 during RSV infection. PLoS One. 2008 Oct 7;3(10):e3352
Haeberle HA, Dürrstein C, Rosenberger P, Hosakote YM, Kuhlicke J, Kempf VA, Garofalo RP, Eltzschig HK
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HIF-dependent repression of nucleoside transporter ENT2 attenuates mucosal inflammation during intestinal hypoxia. Gastroenterology 2009 Feb;136(2):607-18
Rosenberger P, Morote-Garcia JC, Nivillac N, Coe IR, Eltzschig HK
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Hypoxia-inducible-factor dependent induction of Netrin-1 dampens inflammatory hypoxia. Nature Immunology – 2009 Feb;10(2):195-202
Rosenberger P, Schwab JM, Mirakaj V, Masekowsky E, Mager A, Morote-Garcia JC, Unertl K, Eltzschig HK
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Inflammatory Repression of Vasodilator Phosphostimulated Protein (VASP) reduces Pulmonary Barrier Function during Acute Lung Injury. FASEB J. 2009 Dec;23(12):4244-55
Henes J, Schmit M, Mirakaj V, Karhausen J, Morote-Garcia JC, Faigle M, Walter U, Colgan SP, Rosenberger P
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A(2B) adenosine receptors protect against sepsis-induced mortality by dampening excessive inflammation. J Immunol. 2010 Jul 1;185(1):542-50
Csóka B, Németh ZH, Rosenberger P, Eltzschig HK, Spolarics Z, Pacher P, Selmeczy Z, Koscsó B, Himer L, Vizi ES, Blackburn MR, Deitch EA, Haskó G
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Cathepsin X is secreted by human osteoblasts, digests CXCL-12 and impairs adhesion of hematopoietic stem and progenitor cells to osteoblasts. Haematologica, 95: 1452-1460, 2010
Staud et al.
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Netrin-1 dampens Pulmonary Inflammation during Acute Lung Injury. American Journal of Respiratory and Critical Care Medicine, 2010 Apr 15;181(8):815-24
Mirakaj V, Laucher S, Mielke C, Morote-Garcia JC, Unertl KE, Rosenberger P
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Selective induction of endothelial P2Y6 nucleotide receptor promotes vascular inflammation. Blood. 2010 Dec 20. [Epub ahead of print]
Riegel AK, Faigle M, Zug S, Rosenberger P, Robaye B, Boeynaems JM, Idzko M, Eltzschig HK
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The neuronal guidance protein netrin-1 reduces alveolar inflammation in a model of porcine acute lung injury. Critical Care 2010;14(5):R189
Mutz C, Mirakaj V, Vagts DA, Westermann P, Waibler K, König K, Iber T, Nöldge-Schomburg G, Rosenberger P
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Netrin-1 signaling dampens inflammatory peritonitis. J Immunol 2011 Jan 1;186(1):549-55
Mirakaj V, Gatidou D, Pötzsch C, König K, Rosenberger P
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Phosphorylation of vasodilator-stimulated phosphoprotein (VASP) prevents platelet-neutrophil complex formation and dampens myocardial ischemia-reperfusion injury. Circulation. 2011 May 23. [Epub ahead of print]
Köhler D, Straub A, Weissmueller T, Faigle M, Bender S, Kurz J, Lehmann R, Walter U, Zacharowski K, Rosenberger P
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RGM-A inhibits leukocyte migration and mitigates inflammation. Proc Natl Acad Sci U S A. 2011 Apr 19;108(16):6555-60
Mirakaj V, Brown S, Laucher S, Steinl C, Klein G, Skutella T, Koehler D, Schwab JM, Rosenberger P
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Vasodilator Phospho-stimulated Protein (VASP) protects endothelial barrier function during hypoxia. Inflammation. 2011 May 24. [Epub ahead of print]
Schmit MA, Mirakaj V, König K, Köhler D, Rosenberger P
