Die Steuerung der Migration von Neuralleistenzellen bei Amphibienembryonen (Ambystoma, Xenopus) durch Signalmoleküle (Wachstumfaktoren, Matrixmoleküle) Control of neural crest cell migration via signalling molecules (matrix molecules, growth factors, transcription factors) in amphibian embryos (Ambystoma, Xenop

• Antragsteller: Professor Dr. Hans-Henning Epperlein
• Fachliche Zuordnung: Grundlagen der Biologie und Medizin
• Förderung: Förderung von 1998 bis 2001
• Projektkennung: Deutsche Forschungsgemeinschaft (DFG) - Projektnummer 5111162

Investigation of neural crest cell migration in amphibian embryos (Xenopus laevis and Ambystoma mexicanum, the Mexican axolotl) will be continued. After finishing studies on crest cell migration in the head of axolotl embryos, we will now concentrate on the trunk using the fluorescent dye DiI and the transcription factors AP-2 and slug as crest cell markers. Normal crest cell migration and migration after heterotopic grafting (dark head crest into dark trunk) and heteroplastic transplantation (white head crest into dark trunk) will be studied. This will allow assessment of the routes and timing of crest cell migration and differentiation in the trunk. Furthermore, the influence of signalling molecules on neural crest cell migration will be examined. We will focus on hyaluronan (HA), hyaluronidase and BMP-4/noggin, which have shown positive effects on crest cell migration among various molecules tested during the previous funding period. Finally, the potency of the dorsal somite apex to determine the choice of dispersing crest cells for the lateral (pigment cells) or medial route (neurons, glial cells) will be investigated using suppression subtractive hybridization (SSH). *

• DFG-Verfahren: Schwerpunktprogramme
• Teilprojekt zu: SPP 1049: Molekulare Steuerungsmechanismen der Zellwanderung
• Beteiligte Person: Professor Dr. Herbert Steinbeisser (†)