Analysis platform for molecular mechanisms and cellular functions

• Subject Area: Biochemistry ; Animal Physiology and Biochemistry ; Structural Biology
• Term: since 2023
• Project identifier: Deutsche Forschungsgemeinschaft (DFG) - Project number 514177501

A binding analysis device based on microscale thermophoresis contributes an innovative approach to the characterisation of interactions involving proteins, a multi-mode detection device for UV/Vis, fluorescence and luminescence allows a variety of tests in new or improved form, an automated patch clamp system and a system for solid-supported membrane (SSM)-based electrophysiology for high-resolution transporter studies provide the equipment basis for expanding research on membrane transport processes. As potential applications of the analysis platform, research projects on the functional analysis of atypical isoforms of epithelial sodium channels and on aminoacylases are outlined as examples.

• DFG Programme: Major Instrumentation Initiatives
• Major Instrumentation: Automat. Patch-Clamp System + System für Solid-supported membrane (SSM) ; Microscale Thermophorese ; Multi-Modus Mikroskop für UV/Vis, Fluoreszenz und Lumineszenz
• Instrumentation Group: 1390 Sonstige Geräte zur thermischen Trennung und zur Chromatographie (außer 130-138) ; 3590 Sonstige Geräte für Gewebe- und Zelluntersuchung ; 5042 Mikroskope für Hochdurchsatz und Screening
• Applicant Institution: Hochschule Bonn-Rhein-Sieg
• Leader: Professor Dr. Jörn Oliver Sass
• Participating Persons: Professor Dr. Mike Althaus; Professor Dr. Matthias Preller